| 摘要: |
| 目的：观察153钐氯屈膦酸二钠（153Smclodronate）在原位人骨肉瘤裸鼠动物模型中的疗效。方法：以人骨肉瘤细胞系HOS8603接种SCID小鼠皮下，成瘤后经BALB/c裸鼠胫骨移植传代，再接种裸鼠胫骨建立原位人骨肉瘤裸鼠模型，测定153Smclodronate的生物分布，并通过外观、瘤体质量、钼靶X线片、组织学观察和生存期与153SmEDTMP、clodronate对照进行疗效观察。结果：注射后24 h生物分布示瘤骨分布远高于其他组织，放射性计数比瘤骨/血=4 627.11，瘤骨/正常骨=10.81，正常骨/肝脏=8.41。153Smclodronate治疗组较对照组溶骨反应轻，生存期显著延长，但是瘤体质量没有明显差异。结论：153Smclodronate浓聚于瘤骨，在原位人骨肉瘤裸鼠模型中的疗效较好，有进一步实验研究和临床应用的前景。 |
| 关键词: 钐 153 氯屈膦酸二钠 骨肉瘤 移植 模型，动物 |
| DOI：10.3724/SP.J.1008.2007.01094 |
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| 基金项目: |
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| Therapeutic effect of 153sm clodronate on nude mice orthotopically transplanted with human osteosarcoma |
| LUO Xuyao1*，SHEN Qian2，MIAO Jisheng3，CHEN Jian4，XU Jianming5，JI Fang6 |
| (1. Department of Orthopedics, No. 411 Hospital of PLA, Shanghai 200081, China; 2. Department of Laboratory Diagnosis, Changhai Hospital, Second Military Medical University, Shanghai 200433; 3. Department of Immunity, Shanghai First Pulmonary Hospital, Shanghai 200433; 4. Department of Orthopedics, People’s Hospital of Putuo District, Shanghai 200060; 5. Department of Organic Chemistry, School of Pharmacy,Second Military Medical University, Shanghai 200433; 6. Department of Orthopedics, Changhai Hospital, Second Military Medical University, Shanghai 200433) |
| Abstract: |
| Objective：To observe the therapeutic effect of 153Smclodronate on nude mice orthotopically transplanted with human osteosarcoma. Methods: Severe combined immune deficiency (SCID) mice were subcutaneously inoculated with human osteosarcoma cell line HOS8603. The formed tumors were then transplanted into BALB/c nude mice at the tibia for passage; the tumors were harvested for establishing orthotopical mice model of human osteosarcoma. Mice treated with 153SmEDTMP and clodronate group served as controls. The distribution of 153Smclodronate was determined 24 h later. The appearance, tumor weights, Xray examination, histological observation, and survival of mice were compared between the 3 groups. Results: It showed that the distribution of 153Smclodronate in the lesion bone was much higher than that in other organs, with the radioactive ratio of lesion bone to blood being 4 627.11, lesion bone to normal bone being 10.81, and normal bone to liver being 8.41. 153Smclodronate treated mice had a slighter osteolytic reaction and a longer survival period, but the tumor mass was not significantly different compared with those of the control group. Conclusion: 153Smclodronate gathers at the lesion bone and it has good therapeutic effect on the mice model of human oseosarcoma, making it a promising agent for treatment of bone tumor. |
| Key words: samarium 153 clodronate osteosarcoma transplantation models,animal |
