| 摘要: |
| 目的：研究不同年龄组患者颅脑损伤后脑挫裂伤组织中凋亡相关蛋白Bcl2、 Bax的表达差异，探讨年龄因素对颅脑损伤患者预后影响的分子生物学机制。 方法：收集临床脑挫裂伤患者手术切除的脑挫裂伤组织，并随机选取15例颞叶癫或血管畸形手术患者颞叶皮质脑组织作为对照。应用免疫组化和计算机显微图像分析技术，比较老年组(≥60岁)、成年组(19～59岁)和未成年组(≤18岁)患者脑损伤后3～9 h挫裂伤组织中Bcl2、Bax蛋白表达水平的差异。结果：脑损伤后Bcl2、Bax蛋白在脑挫裂伤组织中的表达与对照标本相比明显增强(P<0.05)；老年组患者脑挫裂伤组织中Bcl2/Bax蛋白表达比率显著低于未成年组和成年组(P<0.05)；成年组患者脑挫裂伤组织中Bcl2/Bax蛋白表达比率显著低于未成年组 (P<0.05)。 结论：年龄因素可影响创伤性颅脑损伤后患者脑组织中Bcl2、Bax蛋白的表达，老年颅脑损伤恢复不良可能与Bcl2/Bax蛋白表达比率降低有关，而未成年颅脑损伤患者预后较好可能与Bcl2/Bax蛋白表达比率较高有关。 |
| 关键词: 颅脑损伤 年龄 Bcl 2 Bax |
| DOI：10.3724/SP.J.1008.2007.01113 |
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| 基金项目:南京军区福州总医院院内基金(YN200307). |
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| Expression of Bcl 2 and Bax protein in contusive brain tissue in patients of different ages |
| ZHUANG Zhijun1, GAO Jinxi2*, WANG Rumi2,WANG Shousen2,LIN Ruisheng1, LI Rong1, WANG Weiwei1 |
| (1.Department of Neurosurgery, Zhangzhou Municipal Hospital Affiliated to Fujian Medical University, Zhangzhou 363000, China; 2. Department of Neurosurgery, Fuzhou General Hospital, PLA Nanjing Military Area Command, Fuzhou 350025) |
| Abstract: |
| Objective：To study the influence of different ages on expression of Bcl2 and Bax protein in the contusive brain tissues after traumatic brain injury (TBI), so as to probe into the molecular mechanism of the age influence on the prognosis of TBI patients. Methods: The expression of Bcl2 and Bax protein in contusive and lacerated brain tissue ( within 3 h to 9 h after trauma) in aged group (≥ 60 years), adult group (1959 years) and nonadult group (≤18 years) was analyzed and compared by immunohistochemistry method and computer micrograph analysis. The temporal lobe samples of 15 patients with temporal epilepsy or vascular deformity were taken as control. Results: The expression of NGF, Bcl2 and Bax protein in the contusive and lacerated brain tissue was higher in the 3 TBI groups than that in the control group. The ratio of Bcl2/Bax expression in aged group was significantly lower than those in the adult and nonadult groups (P<0.05); and the ratio of Bcl2/Bax expression in the adult group was significantly lower than that in the nonadult group (P<0.05). Conclusion: The expression of Bcl2 and Bax protein after TBI is influenced by the age of sufferers; the decrease of Bcl2/Bax ratio might be associated with the neurological deficits after TBI in aged patients and the higher ratio of Bcl2/Bax might be associated with the better prognosis of TBI in nonadult patients. |
| Key words: craniocerebral trauma age Bcl 2 Bax |
