Objective：To observe the expression of cellular FLICE-Iike inhibitory protein long form （cFLIPL） mRNA in colon cancer and to assess its relationship with the biological behavior and prognosis of colon cancer. Methods： Expression of cFLIPL mRNA was examined in 86 colon cancer samples and their corresponding normal tissues by semi-quantitative RT-PCR technique. Expression of CEA and P53 in the colon cancer samples was assayed by SABC immunohistochemistry staining. The plasma levels of CEA and CA19-9 were determined by microparticle enzyme-linked immunoassay （MEIA） before operation. Colon cancer tissues were divided into cFLIPL mRNA high expression group and low expression group based on the average value of all the 86 patients. The relationship between the high expression of cFLIPL mRNA and the different clinical features and pathological characters was determined. We also compared the 5-year accumulative survival rates of the 2 groups. Results： The expression of cFLIPL mRNA in the colon cancer tissues was significantly higher than that in the matched normal tissues （0.59±0.10 vs 0.36±0. 10, P〈0.05）. Fifty-six （65. 1%） patients out of 86 had high expression of cFLIP.., mRNA. The cFLIPL mRNA high expression rates in Dukes B,C and D stage patients had an increasing tendency, being 55.3 %,67.5 % and 100%, respectively （P〈0.05）. The postoperative 5-year accumulative survival rate in cFLIPL mRNA high expression group was lower than that in the low expression group （41.1% vs 56.70%, P〈0.05）. There was no significant correlation between cFLIPL mRNA high expression and clinical features/pathological characters other than Dukes stages in the present cohort. Conclusion.. cFLIPL may be related to the clinical staging and prognosis of colon cancer. Detection of cFLIPL mRNA expression in colon cancer tissues can help to fully understand the overall condition of patients, guide treatment, and predict prognosis.