| 摘要: |
| 目的：研究肥胖(ob)基因3′-末端区微卫星多态性与上海地区汉族人群肥胖的关系。方法：运用PCR法分析232例上海地区汉族人群(包括130例肥胖组及102例对照组)的ob基因3′-末端区的微卫星多态性的基因型,比较不同基因型身高、体质量、腰围、臀围、体质量指数、腰/臀比、血糖、血胰岛素、血脂和尿酸的差异。结果：肥胖组和对照组的基因型频率(Ⅰ/Ⅰ 0.115,Ⅰ/Ⅱ 0.331,Ⅱ/Ⅱ 0.554 vs Ⅰ/Ⅰ 0.059,Ⅰ/Ⅱ 0.333,Ⅱ/Ⅱ 0.608)和等位基因频率(Ⅰ 0.281,Ⅱ 0.719 vs Ⅰ 0.225,Ⅱ 0.775)无显著差异；在肥胖组中,携带ob基因(Ⅰ/Ⅰ+Ⅰ/Ⅱ)基因型的患者腰臀比较携带Ⅱ/Ⅱ基因型者大(0.91±0.05 vs 0.86±0.03,P=0.047)。Logistic回归分析证实,腰臀比与ob基因3′-末端区的微卫星多态性独立相关(P=0.042)。结论：Ob基因3′-末端区微卫星多态性与上海地区汉族人群中心性肥胖相关。 |
| 关键词: 汉族 肥胖 肥胖基因 微卫星多态性 |
| DOI：10.3724/SP.J.1008.2007.00933 |
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| 基金项目: |
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| Correlation between microsatellite polymorphisms in ob gene 3′ flanking region with overweight and obesity in Chinese Han ethnicity in Shanghai |
| LU Jin,ZOU Da-jin* |
| (Department of Endocrinology,Changhai Hospital,Second Military Medical University,Shanghai 200433,China) |
| Abstract: |
| Objective：To investigate the correlation between the microsatellite polymorphisms in ob gene 3′flanking region with obesity in Chinese Han ethnicity in Shanghai.Methods: The genotypes of polymorphisms in ob gene 3′flanking region were determined by PCR in 232 Chinese Han subjects (including 130 obese patients and 102 normal controls).The clinical data,including height,weight,waist circumference,hip circumference,body mass index,waist-to hip ratio (WHR),blood glucose,serum insulin,blood lipids,and uric acid,were compared between the 2 groups.Results: No significant difference of genotype frequency (Ⅰ/Ⅰ 0.115,Ⅰ/Ⅱ 0.331,Ⅱ/Ⅱ 0.554 vs Ⅰ/Ⅰ 0.059,Ⅰ/Ⅱ 0.333,Ⅱ/Ⅱ 0.608) and allele frequency (Ⅰ 0.281,Ⅱ 0.719 vs Ⅰ 0.225,Ⅱ 0.775) of ob gene 3′flanking region was observed between the 2 groups.In obese group,patients with Ⅰ+Ⅱ genotype was associated with increased WHR compared with those with Ⅱ/Ⅱ genotype (0.91±0.05 vs 0.86±0.03,P=0.047).Logistic regression analysis showed that WHR was independently correlated with ob gene polymorphisms (P=0.042).Conclusion: Polymorphisms in ob gene 3′ flanking region are correlated with central obesity in obese patients of Chinese Han ethnicity in Shanghai. |
| Key words: Han nationality obesity ob gene microsatellite polymorphism |
