| 摘要: |
| 目的：建立白念珠菌总蛋白质分步提取分离的方法。方法：利用四种溶解能力不同的溶液分步提取白念珠菌总蛋白，定量后，分别进行一维等电聚焦分离和二维聚丙烯酰胺凝胶电泳分离。结果：成功分步提取获得白念珠菌不同溶解度的蛋白，得到各步提取蛋白的二维凝胶电泳图谱。结论：四步法提取白念珠菌总蛋白质是研究白念珠菌蛋白质组的有效方法，为进一步研究白念珠菌耐药机制以及抗真菌药物作用机制奠定基础。 |
| 关键词: 白念珠菌 蛋白质组 四步法 电泳，凝胶，双向 |
| DOI：10.3724/SP.J.1008.2007.00999 |
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| 基金项目:国家自然科学基金（30630071,30672626）;上海市基础研究重点项目（04JC14003） |
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| Establishment of a “Four-step method” for extraction and separation of Candida albicans total proteins |
| XU Yi1,YAN Lan1,ZHANG Jun-dong2,JIANG Yuan-ying* |
| (1.Department of Pharmacology,School of Pharmacy,Second Military Medical University,Shanghai 200433,China; 2. Department of Dematology,Changhai Hospital,Second Military Medical University,Shanghai 200433) |
| Abstract: |
| Objective：To introduce a “Four-step method” for extraction and separation of Candida albicans total proteins.Methods: Proteins of C. albicans were extracted step-by-step with 4 kinds of solutions with different solubilities. After quantification,the protein samples were separated by isoelectric focusing electrophoresis and then by SDS-PAGE. Results: Proteins with different solubilities were successfully extracted step-by-step from C. albicans and were separated by two-dimensional gel electrophoresis. Conclusion: The “Four-step method” for extraction of C. albicans proteins is an effective approach to study C. albicans membrane proteome and lays a foundation for further investigation of the mechanisms of antifungal agents and drug resistance in C. albicans. |
| Key words: Candida albicans proteome four-step method electrophoresis,gel,two-dimensional |
