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  • 邱彦,刘静,刘梅,司梁宏,茆玉国,陆瑜.选择性环氧合酶-2抑制剂NS-398体外抑制结肠癌细胞增殖和侵袭[J].第二军医大学学报,2008,29(8):0925-0928    [点击复制]
  • QIU Yan,LIU Jing,LIU Mei,SI Liang-hong,MAO Yu-guo,LU Yu.Inhibitory effect of selective cyclooxygenase-2 inhibitor NS-398 on proliferation and invasion of human colorectal cancer cells[J].Acad J Sec Mil Med Univ,2008,29(8):0925-0928   [点击复制]
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选择性环氧合酶-2抑制剂NS-398体外抑制结肠癌细胞增殖和侵袭
邱彦,刘静,刘梅,司梁宏,茆玉国,陆瑜
0
(解放军第454医院药剂科,南京 210002)
摘要:
目的:观察环氧合酶-2(COX-2)抑制剂NS-398对结肠癌细胞增殖、侵袭的影响,探讨COX-2作为结肠癌治疗靶标的可行性。方法:用RT-PCR和Western印迹法检测结肠癌细胞CW-2、COLO-320中COX-2基因及蛋白的表达。MTT法观察NS-398对COLO-320细胞增殖的抑制作用;体外细胞侵袭试验检测NS-398对细胞侵袭能力的影响;Western印迹检测NS-398对基质金属蛋白酶2(MMP-2)表达的影响。结果:结肠癌细胞株CW-2、COLO-320中COX-2均有阳性表达。NS-398能抑制结肠癌细胞株COLO-320的生长,抑制作用具有时间、浓度依赖性。细胞侵袭试验表明,NS-398体外能抑制结肠癌细胞株COLO-320的侵袭。Western 印迹结果表明NS-398可以抑制COLO-320中MMP-2的表达。结论:COX-2抑制剂NS-398体外可显著抑制结肠癌细胞的生长、侵袭,COX-2可作为结肠癌治疗的靶点。
关键词:  环氧合酶-2抑制剂  结肠肿瘤  肿瘤侵袭  基质金属蛋白酶2
DOI:10.3724/SP.J.1008.2008.00925
投稿时间:2008-02-27修订日期:2008-04-01
基金项目:
Inhibitory effect of selective cyclooxygenase-2 inhibitor NS-398 on proliferation and invasion of human colorectal cancer cells
QIU Yan,LIU Jing,LIU Mei,SI Liang-hong,MAO Yu-guo,LU Yu
(Department of Pharmacy,No454 Hospital of PLA,Nanjing 210002,China)
Abstract:
Objective:To investigate the effect of NS-398,a selective cyclooxygenase-2 (COX-2 ) inhibitor,on the proliferation and invasion of human colon carcinoma cells in vitro,so as to determine the possibility of COX-2 as a new target for treatment of colon carcinomaMethods: The expression of COX-2 in colorectal cancer cells (CW-2,COLO-320) was detected by RT-PCR and Western blottingCOLO-320 cell proliferation was measured by MTT after treatment with NS-398Cell invasion ability was measured using migration and invasion chamber systemsWestern blotting assay was used to examine the influence of NS-398 on MMP-2 expressionResults: Our results showed that CW-2,COLO-320 cells expressed COX-2 mRNA and proteinNS-398 inhibited the proliferation of COLO-320 cells in a time- and concentration-dependent mannerInvasion test showed that NS-398 inhibited the migration and invasion of COLO-320 cellsWestern blotting revealed that NS-398 inhibited the expression of MMP-2 in COLO-320 cellsConclusion: The selective COX-2 inhibitor NS-398 can inhibit COLO-320 cell proliferation and invasion,indicating COX-2 may serve as a new target for colon carcinoma treatment
Key words:  specific cyclooxygenase-2 inhibitor  colonic neoplasms  neoplasm invasiveness  matrix metalloproteinase-2