| 摘要: |
| 研究促性腺激素释放激素(GnRH)类似物曲谱瑞林(triptorelin)体外逆转卵巢癌细胞顺铂耐药的效果，并探讨其作用机制。方法：体外建立卵巢癌顺铂耐药细胞系模型OVCAR-3/CDDP； MTT法检测单独使用曲谱瑞林或顺铂及两药联合使用时对OVCAR-3/CDDP细胞的抑制作用；流式细胞术检测不同加药组细胞的表皮生长因子受体(epidermal growth factor receptor,EGFR)表达改变。结果：(1)卵巢癌OVCAR-3/CDDP细胞耐药指数为13.42；(2)顺铂与曲谱瑞林联合化疗逆转倍数为3.91；(3)OVCAR-3/CDDP细胞EGFR表达在两药联合处理组降低最明显。结论：曲谱瑞林能部分逆转OVCAR-3/CDDP细胞对顺铂的耐药性，且此作用可能与下调细胞EGFR表达有关。 |
| 关键词: 曲谱瑞林 卵巢肿瘤 顺铂 耐药性 |
| DOI：10.3724/SP.J.1008.2008.00395 |
| 投稿时间：2008-03-28修订日期：2008-03-28 |
| 基金项目: |
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| GnRH analog resensitizes cisplatin-resistant human ovarian cancer cells |
| WANG Dan,HUI Ning*,WU Dong |
| (Department of Gynecology and Obstetrics，Changhai Hospital，Second Military Medical University，Shanghai 200433, China) |
| Abstract: |
| Objective：To study the effect of GnRH analog triptorelin in resensitizing cisplatin-resistant human ovarian cancer cells and to discuss the related mechanism. Methods: Cisplatin-resistant human ovarian cancer cell line OVCAR-3/CDDP was established in vitro. MTT assay was used to assess the inhibitory effects of triptorelin, cisplatin alone or a combination of both on OVCAR-3/CDDP cells. Flow cytometry was employed to observe the expression changes in epidermal growth factor receptor (EGFR) in different groups. Results: The drug restant index of OVCAR-3/CDDP cells was 13.42. The resensitizing fold of cisplatin combined with triptorelin was 3.80. The expression of EGFR had the most prominent decrease in OVCAR-3/CDDP cells in the combination group. Conclusion: Triptorelin can partially resensitize cisplatin-resistant OVCAR-3/CDDP cells, which might be related to the down-regulation of EGFR. |
| Key words: triptorelin ovarian neoplasms cisplatin drug tolerance |
