| 摘要: |
| 目的:研究多棘海盘车正丁醇萃取部位的化学成分。 方法:利用溶媒萃取和多种色谱法,对多棘海盘车的正丁醇萃取部位化学成分进行分离,根据理化性质和现代波谱技术鉴定化合物的化学结构,并测试了其中5个化合物的生物活性。 结果:分离并鉴定了5个化合物,分别为多羟基甾体皂苷amurensoside D(Ⅰ)、色氨酸(Ⅱ)和3个硫酸甾醇,包括3β-O-硫酸酯化-6α,23β-二羟基-胆甾-9(11)-23-羰基-硫酸盐(Ⅲ)、3β-O-硫酸酯化-6α-羟基-胆甾-9(11),20(22)-二双键-23-羰基-硫酸盐(Ⅳ)和3β-O-硫酸酯化-6α-羟基-麦角甾-9(11)-双键-23-羰基-硫酸盐(Ⅴ)。 结论:化合物Ⅱ是首次从多棘海盘车中得到,化合物Ⅲ和Ⅴ对稻瘟霉菌P-2b显示较强的药理活性。 |
| 关键词: 多棘海盘车 皂苷 硫酸甾醇 |
| DOI：10.3724/SP.J.1008.2008.01479 |
| 投稿时间：2008-05-05修订日期：2008-08-11 |
| 基金项目:国家高新技术研究发展计划(“863”计划)(2006AA09Z423)；长征医院“三重三优”学科人才建设计划. |
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| Study on chemical constituents of Starfish Asterias amurensis |
| CHENG Ping1,TANG Hua2,PIAO Shu-juan1,LU Yi1,WANG Zeng-lei2,LIN Hou-wen1*,LIN Hou-wen |
| (1.Department of Pharmacy,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China*2.Department of Marine Drugs,School of Pharmacy,Second Military Medical University,Shanghai 200433) |
| Abstract: |
| Objective:To investigate the chemical constituents in the BuOH extract of Asterias amurensis.Methods: The compounds were obtained by a combination of extraction and purification with suitable solvent and multiple column chromatographies; their structures were determined based on spectral analysis and physicochemical properties.The bioactivities of 5 compounds were tested.Results: Five compounds were isolated and identified,and they were amurensoside D(Ⅰ),tryptophan Ⅱ,and 3 sulfated sterols,including 3β-O-sulfated-6α,20β-diol-cholest-9(11)-en-23-one sodium salt(Ⅲ),3β-O-sulfated-6α-ol-cholesta-9(11),20(22)-dien-23-one sodium salt(Ⅳ),and 3β-O-sulfated-6α-ol-ergost-9(11)-en-23-one sodium salt(Ⅴ).Conclusion: TryptophanⅡ is obtained from this species for the first time; compoud Ⅲ and compound Ⅴ exhibit potent inhibitory activity against Pyricularia oryzae P-2b. |
| Key words: Asterias amurensis saponin sulfated sterols |
