| 摘要: |
| 目的:研究PTEN基因在旁效应细胞中的表达变化。方法:采用未照射细胞与直接照射细胞按11共同培养,以及将直接照射细胞培养液离心加入未照射细胞中培养形成2种旁效应细胞模型,对比PTEN mRNA及蛋白表达在2种旁效应细胞、直接照射细胞、未照射细胞中的变化。结果:直接照射的PTEN 蛋白表达与照射剂量有关,照射剂量越大其下调越为明显;PTEN蛋白表达下调程度与细胞的处理方式有关,以受照细胞培养液培养的旁效应细胞表达下调最为显著,且2种旁效应细胞中PTEN蛋白的表达下调程度均高于直接受照细胞(P<0.01)。结论:PTEN蛋白表达与低剂量电离辐射生物学效应及电离辐射旁效应的发生发展密切相关。 |
| 关键词: PTEN 电离辐射 旁效应 细胞增殖 细胞周期 |
| DOI：10.3724/SP.J.1008.2009.0137 |
| 投稿时间：2008-05-05修订日期：2008-10-23 |
| 基金项目:国家自然科学基金(10575130). |
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| Changes of PTEN gene expression in radiation induced bystander cells |
| YANG Jian,HAN Ling*,GAO Xian |
| (Department of Radiation Medicine,Faculty of Naval Medicine,Second Military Medical University,Shanghai 200433,China) |
| Abstract: |
| Objective:To study the changes of PTEN gene expression in radiationinduced bystander cells.Methods: The irradiated cells and nonirradiated cells were cocultured in a ratio of 11.The culture medium of irradiated cells was used to culture nonirradiated cells to create two bystander cell models. The expression of PTEN mRNA and protein in the bystander cells,irradiated cells,nonirradiated cells was compared.Results: The protein expression of PTEN in the irradiated cells was negatively associated with the irradiation dose.The downregulation degree of PTEN protein was related to the different treatments of cells.The most prominent downregulation was found in the bystander cells cultured with medium of irradiated cells.The downregulation of PTEN protein in the two kinds of bystander cells was more severe than that in the irradiated cells(P<0.01).Conclusion: PTEN protein expression can be influenced by low dose radiation and the bystander effect of radiation. |
| Key words: PTEN ionizing radiation bystander effect cell proliferation cell cycle |
