| 摘要: |
| 目的:研究妊娠期大鼠接触糖皮质激素(GCs)对子代大鼠海马组织血清和糖皮质激素调节蛋白激酶(SGK)表达的影响，并初步探讨其可能原因。方法:根据大鼠发情周期进行合笼，次晨阴道涂片发现精子作为受孕0.5 d。孕鼠随机分为对照组和地塞米松组，每组10只。地塞米松组大鼠在妊娠第14 日起皮下注射地塞米松 0.1 mg/ (kg·d) ,对照组注射等量生理盐水。取出生后1、7和42 d的子代大鼠海马组织,采用real-time PCR法检测SGK和糖皮质激素受体(GR)mRNA表达情况。结果:地塞米松组子代出生时体质量明显低于对照组,随后出现追赶性生长；地塞米松组子代在出生后1、7、42 d，其海马组织SGK 和GR mRNA表达较对照组明显减少(P<0.05)。结论:妊娠晚期接触GCs可使子代大鼠海马SGK和GR的基础表达降低。 |
| 关键词: 妊娠 糖皮质激素 印痕效应 海马 SGK |
| DOI：10.3724/SP.J.1008.2008.01495 |
| 投稿时间：2008-06-27修订日期：2008-09-22 |
| 基金项目: |
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| Glucocorticoid exposure of pregnant rats influences hippocampus SGK expression in rat offsprings |
| CONG Bin-hai,LI Yue-hua,SUN Ting-ting,ZHU Xiao-yan,WEN Jian-nan,HU Jia,SHENG Hui,NI Xin*,NI Xin |
| (Department of Physiology,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433,China) |
| Abstract: |
| Objective:To observe the effect of glucocorticoid exposure of pregnant rats on hippocampus SGK expression in rat offsprings,and to explore the related mechanism.Methods:Pregnancy was determined by examining vaginal smear in the morning of cohabiting.Pregnant rats were divided into control group and dexamethasone group,with each group containing 10 rats.Dexamethasone group was subcutaneously injected with dexamethasone(0.1 mg/ \[kg·d\] ) and control group was injected with normal saline.The hippocampuses of rat offsprings were collected 1 d，7 d,and 42 d after birth.Real-time PCR was used to determine SGK and GR mRNA expression.Results:The body weight of rat offsprings in the dexamethasone group was obviously lower than that in the control group at birth,then those in the dexamethasone group had a rapid growth.Prenatal exposure to dexamethasone significantly decreased the expression of SGK and GR mRNA in the hippocampuses of rat offsprings (1 d,7 d and 42 d after birth) compared with control group (P<0.05).Conclusion:Prenatal exposure to glucocorticoid in pregnancy stage can decrease the hippocampus expression of SGK and GR in rat offsprings. |
| Key words: pregnancy glucocorticoids imprinting effect hippocampus SGK |
