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  • 沈淑蓉1,施俊义1*,沈贤2 ,黄关立2 ,薛向阳2.Let-7c在乳腺浸润性导管癌中的表达及临床意义[J].第二军医大学学报,2009,30(4):400-403    [点击复制]
  • SHEN Shu-rong1, SHI Jun-yi1*, SHEN Xian2, HUANG Guan-li2, XUE Xiang-yang2.Expression of Let-7c in invasive ductal carcinoma of breast cancer and its clinical significance[J].Acad J Sec Mil Med Univ,2009,30(4):400-403   [点击复制]
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Let-7c在乳腺浸润性导管癌中的表达及临床意义
沈淑蓉1,施俊义1*,沈贤2,黄关立2,薛向阳2
0
(1.第二军医大学长海医院普通外科,上海 200433;2.温州医学院附属第一医院普通外科,温州 325000)
摘要:
目的:探讨Let-7c在乳腺浸润性导管癌中的表达情况及与各种临床病理特征的关系。方法:采用茎环RT-PCR方法检测40例乳腺浸润性导管癌及其对应的正常乳腺组织中Let-7c的表达,分析Let-7c表达与临床分期、淋巴结转移、增殖指数等临床病理特征的关系。结果:用茎环RT-PCR方法检测Let-7c表达的敏感性和特异性良好; Let-7c在乳腺癌组织中的表达显著低于正常组织(P<0.05);Let-7c的表达与乳腺癌的增殖指数有关(P<0.05),与雌孕激素受体状态亦有关,与是否绝经、乳腺癌的TNM分期、淋巴结转移未见显著统计学意义(P>0.05)。在高增殖指数、雌孕激素受体阴性的肿瘤组Let-7c表达明显降低。结论:Let-7c在乳腺浸润性导管癌中的表达异常,可能和乳腺癌的发生和发展有关,有望成为乳腺癌新的预后指标及治疗靶点。
关键词:  Let-7c基因  微MicroRNA  乳腺肿瘤  乳腺导管癌
DOI:10.3724/SP.J.1008.2009.0400
投稿时间:2008-09-07修订日期:2009-03-14
基金项目:温州市卫生局课题(2007010).
Expression of Let-7c in invasive ductal carcinoma of breast cancer and its clinical significance
SHEN Shu-rong1, SHI Jun-yi1*, SHEN Xian2, HUANG Guan-li2, XUE Xiang-yang2
(1.Department of General Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433,China;2.Department of General Surgery, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000)
Abstract:
Objective:To investigate the expression of Let-7c in patients with invasive ductal carcinoma of the breast cancer and its correlation with the clinicopathologic features of the patients. Methods: The expression of Let-7c was examined in 40 tissues of invasive ductal carcinoma of the breast cancer and their matched non-tumor adjacent tissue specimens by stem-loop real-time RT-PCR. The correlation of the expression with the clinicopathological features of breast cancer, such as the clinical staging, lymphatic metastasis, and proliferation index, was analyzed. Results: The stem-loop realtime RT-PCR was sensitive and specific in detecting Let-7c expression. Expression of Let-7c in breast cancer tissues was significantly lower than that in the adjacent normal tissues(P<0.05). Down-regulation of Let-7c expression was associated with higher proliferation index (Ki-67>10%) (P=0.030) and the status of Estrogen/Progesterone receptor (P=0.040/0.034)in breast cancer patients. There was no significant association of Let-7c expression with menopause condition, breast cancer TNM clinical stage, or lymphatic metastasis(P>0.05). Conclusion: Our results suggest Let-7c may play an important role in the development of breast cancer and the level of Let-7c expression may be a potential parameter in forecasting the progression and prognosis of breast cancer patients.
Key words:  Let-7c gene  MicroRNAs  breast neoplasms  breast ductal carcinoma