Objective:Currently most researches concerning electret transdermal patches are performed by one-factor experimental design with relatively poor efficiency. To analyze the influencing factors in a more systematical and effective manner,we put forward an optimized design for in vitro experiment with electret transdermal patches. Methods:Due to the great number and various levels of factors impacting the charge storage stability of electrets,we combined uniform design and orthogonal design to optimize the screening for the stable transdermal patch. In the following in vitro study,we optimized the design of percutaneous experiments to screen for charge polarity and penetration enhancers using a two-factor experimental design. And using an orthogonal experimental design,we further studied the influence of different levels of main drug,surface potential and penetration enhancer on transdermal absorption.Conclusion:The present design,taking into consideration of various factors and using multi-factor experimental design,can more effectively analyze data,reduce workload,and is feasible.