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  • 张颖玮,时多,熊锡山,胡惠民,梅长林*.罗格列酮干预对老年SD大鼠氧自由基及相关生化指标的影响[J].第二军医大学学报,2009,30(2):183-186    [点击复制]
  • ZHANG Ying-wei,SHI Duo,XIONG Xi-shan,HU Hui-min,MEI Chang-lin*.Effect of Rosiglitazone on oxygen free radical and biochemical parameters in aged SD rats[J].Acad J Sec Mil Med Univ,2009,30(2):183-186   [点击复制]
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罗格列酮干预对老年SD大鼠氧自由基及相关生化指标的影响
张颖玮,时多,熊锡山,胡惠民,梅长林*
0
(第二军医大学长征医院肾内科,上海 200003)
摘要:
目的:观察罗格列酮对衰老大鼠血液生化指标及氧自由基的影响,为将来抗衰老的实验研究提供一定的实验依据。方法:24个月龄(老年)自然衰老SD大鼠为模型,随机分为正常对照组、罗格列酮组、能量限制组,每组10只。罗格列酮组给予罗格列酮4 mg/(kg·d)灌胃,能量限制组给予正常对照组饮食的60%,第12周治疗结束时处死所有动物并检测各组大鼠的血液生化指标及超氧化物歧化酶(SOD)和丙二醛(DA) 的酶活性。结果:各组大鼠血糖值(GLU)、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、肌酐(Scr)、尿素氮(BUN)无明显差异,均在正常范围内。罗格列酮组血胆固醇(CH)较对照组出现下降,但无统计学差异。罗格列酮组及限食组血三酰甘油(TG)均较对照组出现了明显下降(P<0.05)。罗格列酮组及限食组血液SOD及MDA酶活性均较对照组有明显改善。结论:罗格列酮具有调节老年大鼠体内脂质代谢紊乱、减轻氧化损伤的作用,可能起到抗衰老的作用。
关键词:  罗格列酮  衰老  超氧化物歧化酶  丙二醛  三酰甘油
DOI:10.3724/SP.J.1008.2009.0183
投稿时间:2008-09-27修订日期:2008-12-15
基金项目:国家重点基础研究发展计划(“973”计划,2007CB07400)
Effect of Rosiglitazone on oxygen free radical and biochemical parameters in aged SD rats
ZHANG Ying-wei,SHI Duo,XIONG Xi-shan,HU Hui-min,MEI Chang-lin*
(Department of Nephrology,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China)
Abstract:
Objective:To observe the effect of Rosiglitazone (RGTZ) on oxygen free radical and biochemical parameters of aged rats,so as to establish a basis for anti-aging study.Methods:The 24-month-old naturally aged SD rats were randomly divided into the control group (CONT),the RGTZ group and the caloric restriction group(CR).The RGTZ group was given intragastric administration of RGTZ (4 mg·kg-1·d-1) and the CR group was given 60% of the diet that of the CONT group.The rats were killed 12 weeks later and the serum malondialdehyde(MDA) content,superoxide dismutase(SOD) activity and the blood biochemistry parameters were examined.Results:The blood glucose (GLU),aspartate transaminase(AST), alamine transaminase(ALT),serum creatinine (SCr),and blood urea nitrogen(BUN) were within the normal range in all groups.Compared with the CONT group,blood cholesterol (CH) in RGTZ group was lower,but with no significant difference.The serum triglyceride(TG) of both RGTZ and CONT groups decreased markedly than those in the CONT group(P<0.05).The activities of SOD and MDA were improved in RGTZ and CR group compared with the control group.Conclusion:RGTZ can regulate the lipid metabolism and attenuate the oxidative damage in aged rats,indicating its anti-aging effect.
Key words:  rosiglitazone  aging  superoxide dismutase  malondialdehyde  blood biochemistry  triglycerides