原花青素预处理对体外人源细胞辐射损伤的防护作用
CSTR:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金(30470415,30800284).


Protective effects of proanthocyanidin on radiation-induced injury of human-derived cells
Author:
Affiliation:

Fund Project:

Supported by National Natural Science Foundation of China (30470415,30800284).

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:探讨原花青素(PC)预处理对体外人淋巴母细胞AHH-1和肠腺上皮细胞HIEC电离辐射损伤的防护效果及可能的作用机制。方法:采用CCK-8法检测原花青素预处理对γ射线照射后AHH-1、HIEC细胞存活率的影响,Annexin-Ⅴ/PI染色和流式细胞术分析细胞凋亡及细胞周期的变化,蛋白质印迹法检测细胞Bcl-2蛋白表达变化。结果:PC预处理的细胞辐照后存活率明显升高(P<0.01),4 Gy照射用药组 AHH-1细胞凋亡率(11.78%)显著低于单纯照射组(26.38%,P<0.01),8 Gy照射用药组HIEC细胞凋亡率(5.32%)亦显著低于单纯照射组(12.45%,P<0.01)。PC预处理组AHH-1、HIEC细胞受照射后其Bcl-2蛋白表达下降受到抑制。 结论:PC预处理对细胞的辐射损伤具有较好的防护效果,可能与其促进受损细胞Bcl-2蛋白表达有关。

    Abstract:

    Objective:To investigate the protective effects of proanthocyanidin on radiation-induced injury of AHH-1 and HIEC cells,and to explore the possible molecular mechanism.Methods: CCK-8 assay was applied to examine the survival of AHH-1,HIEC cells after γ-ray irradiation with or without proanthocyanidin pretreatment.Annexin-Ⅴ/PI staining and flow cytometry analysis were used to evaluate the apoptosis and cell cycle of AHH-1 and HIEC cells.The expression of Bcl-2 protein was analyzed by Western blotting assay.Results: Pretreatment with proanthocyanidin significantly increased the cell survival rate after radiation(P<0.01).The apoptosis rates of cells in the proanthocyanidin+radiation group were (11.78% at 4 Gy\[AHH-1\] and 5.32% at 8 Gy\[HIEC\],respectively) greatly lower than those of the single radiation group (26.38% at 4 Gy\[AHH-1\] and 12.45% at 8 Gy\[HIEC\],respectively,P<0.01).Moreover,the inhibition of Bcl-2 protein expression in AHH-1,HIEC cells was attenuated in the proanthocyanidin+radiation group.Conclusion: Proanthocyanidin has satisfactory protective effect on radiation-induced cellular injury; the mechanism may be related to the attenuation of Bcl-2 protein inhibition.

    参考文献
    相似文献
    引证文献
相关视频

分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2009-01-20
  • 最后修改日期:2009-05-20
  • 录用日期:2009-06-12
  • 在线发布日期: 2009-06-18
  • 出版日期:
文章二维码
重要通知
友情提醒: 近日发现论文正式见刊或网络首发后,有人冒充我刊编辑部名义给作者发邮件,要求添加微信,此系诈骗行为!可致电编辑部核实:021-81870792。
            《海军军医大学学报》编辑部
关闭