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| 植物转基因发根的研究方向和应用 |
| 刘娟1,2,李杨1,2,陈万生3,张磊1* |
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| (1.第二军医大学药学院生药学教研室,上海 200433;2.佳木斯大学化学与药学院生药学教研室,佳木斯 154007;3.第二军医大学长征医院药学部,上海 200003) |
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| 摘要: |
| 利用发根农杆菌转化药用植物建立发根培养体系,以其独有的优势可以大规模生产次生代谢物,有效解决天然植化产品资源短缺的问题。同时诱发植物产生毛状根的质粒（Ri质粒）还是植物基因工程理想的载体,可将抗虫、抗病基因或植物次生代谢物合成关键酶基因,进一步整合到植物宿主基因组并得到表达,从而达到改良植物性状、提高次生代谢物含量的目的。转基因发根培养体系的建立为进一步利用遗传代谢工程手段调控植物次生代谢物含量和进行药用活性成分的工业化生产奠定了基础。本文综述了该领域的最新研究进展和相关应用。 |
| 关键词: 植物 发根农杆菌 转基因发根 代谢工程 |
| DOI：10.3724/SP.J.1008.2010.0433 |
| 投稿时间：2009-02-25修订日期：2010-01-28 |
| 基金项目:国家自然科学基金（30600807）. |
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| Transgenic plant hairy root: future and application |
| LIU Juan1,2, LI Yang1,2, CHEN Wan-sheng3, ZHANG Lei1* |
| (1. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China;2. Department of Pharmacognosy, Chemical and Parmacy College, Jiamusi University, Jiamusi 154007, Heilongjiang, China;3. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China) |
| Abstract: |
| Establishment of a hairy root culture system from the medicine plants transformed by the Agrobacterium rhizogenes has unique advantages in large scale production of secondary metabolites and provides an effective solution to the shortage of resources. Meanwhile, root-induced(Ri) plasmid is also an ideal vector for plant genetic engineering. Insect-resistant, disease-resistant genes or genes encoding key enzymes involved in biosynthesis of plant secondary metabolites can be harbored by Ri plasmid and integrated into the host plant genome for expression, thus improving the plant traits and enhancing the secondary metabolite content. Establishment of plant transgenic hairy root culture system has laid a solid foundation for regulating plant secondary metabolites content and for industrial production of pharmaceutical active ingredients by using of genetic metabolic engineering strategies. This paper reviews the latest research advances in this field and the related applications. |
| Key words: plant Agrobacterium rhizogenes transgenic hairy root metabolic engineering |
