| 摘要: |
| 目的:观察有机锗(β-羧乙基锗倍半氧化物,β-carboxyethylgermanium sesquioxide,Ge-132)对佐剂诱导关节炎大鼠炎症组织局部组胺(histamine,HA)及5-羟色胺(5-hydroxytryptamine,5-HT)表达的影响。方法:雄性SD大鼠随机分为6组(n=10)：空白对照组,模型组,低(10 mg/kg)、中(20 mg/kg)、高剂量(40 mg/kg)Ge-132治疗组以及吲哚美辛(IDMT,1 mg/kg)对照组。除空白对照组外其余各组大鼠均在右后足跖皮下注射弗氏完全佐剂(Freund’s complete adjuvant,FCA)0.1 ml制备实验性类风湿性关节炎(RA)大鼠模型。于FCA注射前、注射造模后第3、6、9、12、15、18、21天测大鼠体质量和右足足跖容积,计算体质量增长率和足跖肿胀率。造模第21天处死大鼠,取右后足,测定炎症组织局部HA及5-HT的含量。结果:造模第21天,中、高剂量Ge-132治疗组及IDMT对照组大鼠体质量增长率优于模型组及低剂量组(P<0.01),与空白对照组无统计学差异。低、中、高剂量组大鼠足跖肿胀程度明显低于模型组(P<0.01),但高于IDMT对照组(P<0.01或0.05)；高剂量组肿胀程度明显低于低、中剂量组(P<0.05)。低、中、高剂量组及IDMT对照组大鼠关节炎局部HA含量均显著低于模型组(P<0.01)；但低、中剂量组高于高剂量组、IDMT对照组(P<0.01)。中、高剂量组及IDMT对照组大鼠关节炎局部5-HT含量显著低于模型组和低剂量组(P<0.01)。结论:中、高剂量Ge-132可能通过抑制HA、5-HT等炎症介质的释放显著减轻关节炎大鼠的局部炎症,效果与IDMT相近。 |
| 关键词: 类风湿关节炎 锗 组胺 5-羟色胺 |
| DOI：10.3724/SP.J.1008.2009.0793 |
| 投稿时间：2009-03-06修订日期：2009-05-11 |
| 基金项目:中国营养学会营养科研基金(yj0606). |
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| Inhibitory effect of germanium against local expression of 5-HT and HA in arthritic rats |
| ZHANG Zhe,GUO Miao-li,DING Wen,GUO Jun-sheng* |
| (Faculty of Naval Medicine,Second Military Medical University,Shanghai 200433,China) |
| Abstract: |
| Objective:To investigate the effect of germanium against local expression of 5-hydroxytryptamine (5-HT) and histamine (HA) in adjuvant-induced arthritic rats.Methods: Male SD rats were randomly divided into 6 groups (n=10): blank control group,model group,low(10 mg/kg),medium(20 mg/kg),and high dose(40 mg/kg)β-carboxyethylgermanium sesquioxide (Ge-132) groups,and indomethacin(IDMT,1 mg/kg)control group.Rat model of rheumatoid arthritis (RA) was induced in the later 4 groups by Freund’s complete adjuvant (FCA).The body weights and the paw volumes of rats in all groups were determined before FCA injection and d 3,6,9,12,15,18,and 21 after injection; the rates of weight gain and paw swelling were calculated.The contents of HA and 5-HT in inflammatory tissues were measured at d 21 immediately after rats were beheaded.Results: The rates of weight gains in middle and high dose germanium groups and IDMT group were significantly higher than those in the low dose germanium group and model groups(P<0.01)at d 21,but had no difference with those of blank control group.The rates of paw swelling in the three germanium groups were significantly lower than that of the model group(P<0.01),and higher than that of the IDMT group(P<0.01 or 0.05) ; that of the high germanium group was significantly lower than those of the other 2 germanium groups (P< 0.05).The contents of HA in the local asthmatic tissues in the 3 germanium and IDMT groups were significantly lower than that of the model group,and those of the low and middle germanium groups were significantly higher than those of the higher germanium and IDMT groups (P<0.01).The contents of 5-HT in middle and high germanium and IDMT groups were significantly lower than those of the model group and low germanium(P<0.01).Conclusion: Middle and high dose germanium may relieve local asthmatic inflammation in rats through inhibiting the release of HA and 5-HT,with its effect similar to that of IDMT. |
| Key words: rheumatoid arthritis germanium histamine 5-hydroxytryptamine |
