| 摘要: |
| 目的 探讨人源幼虫巨大致死性基因Hugl-1在卵巢癌中的表达及其临床意义。方法 利用实时荧光定量RT-PCR和原位杂交技术检测31例卵巢癌组织及其相应的癌旁组织中Hugl-1 mRNA的表达量，并结合卵巢癌的临床病理特点进行分析。结果 Hugl-1 mRNA在卵巢癌组织中的表达量高于癌旁卵巢组织\[(0.051±0.029 ) vs (0.026±0.043),P<0.01\]。Hugl-1 mRNA的表达量与卵巢癌患者的年龄、血清CA125、肿瘤病理特征及临床分期无关。结论 Hugl-1在卵巢癌的发生发展中起着一定作用。 |
| 关键词: 卵巢肿瘤 人源幼虫巨大致死基因 基因表达 原位杂交 |
| DOI：10.3724/SP.J.1008.2010.0513 |
| 投稿时间：2009-05-27修订日期：2010-04-14 |
| 基金项目: |
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| Expression of human giant larvae-1 in ovarian cancer and its clinical significance |
| ZHANG Yan*, MAN Xiao-bo, BAI Chen-guang, HUI Ning |
| (Department of Obstetrics and Gynecology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| Objective To study the expression of human giant larvae-1 (Hugl-1) in ovarian carcinoma and its clinical significance. Methods Hugl-1 mRNA expression in 31 ovarian cancer and the corresponding adjacent tissues was examined by real-time fluorescence quantitative RT-PCR and in situ hybridization. Moreover, analysis was done while taking into consideration of the clinicopathologic parameters of ovarian cancer. Results The expression of Hugl-1 mRNA in ovarian cancer tissue was significantly higher than that in the corresponding adjacent tissues (\[0.051±0.029\] vs \[0.026±0.043\],P<0.01); and the expression was not related to patient age, serum CA125 status, pathological characteristics or clinical stages of tumors. Conclusion Hugl-1 may play a role in the development and progresssion of ovarian cancer. |
| Key words: ovarian neoplasms Hugl-1 gene expression in situ hybridization |
