| 摘要: |
| 目的建立同时测定双五胶囊中紫丁香苷、五味子醇甲、五味子甲素、五味子乙素含量的方法。方法HPLC法，采用Diamonsil C18柱(200 mm×4.6 mm，5 μm)，流动相为A：甲醇-乙腈(1∶1)，B∶水，梯度洗脱，0～5 min， 35%～60%A；5～10 min ，60%～70%A；10～50 min， 70%～90%A；50～90 min， 90%A。流速为1 ml/min，柱温：35℃，检测波长220 nm。结果紫丁香苷、五味子醇甲、五味子甲素、五味子乙素浓度分别在1.28～20.40 μg/ml(r=0.999 7)、6.30～100.80 μg/ml(r=0.999 6)、1.20～19.20 μg/ml (r=0.999 8) 和3.75～60.00 μg/ml(r=0.999 6) 范围内呈良好的线性关系。4种成分精密度实验RSD<1%。24 h 内稳定性RSD<1.5%。紫丁香苷、五味子醇甲、五味子甲素、五味子乙素的平均加样回收率分别为99.47%、102.50%、99.21%、 101.86%。结论所建立的方法具有快速、简便、准确等优点,可用于双五胶囊的质量控制。 |
| 关键词: 双五胶囊 紫丁香苷 五味子醇甲 五味子甲素 五味子乙素 高压液相色谱法 |
| DOI：10.3724/SP.J.1008.2010.091 |
| 投稿时间：2009-07-26修订日期：2009-11-24 |
| 基金项目: |
|
| HPLC in simultaneous determination of four components in Shuangwu capsules |
| YE Guang-ming1,JIANG Yun-yun1*,CHEN Yun-hong1,WANG Fa-xiang1,WU Ming-nan2,XIAO Fei2 |
| (1.Departement of Pharmacy,No.101 Hospital of PLA,Wuxi 214044,Jiangsu,China;2.Department of Pharmacy,the First People’s Hospital of Chaohu City,Chaohu 238000,Anhui,China) |
| Abstract: |
| ObjectiveTo establish an HPLC method for simultaneous determination of syringin,schizandrin,deoxyschizandrin and schisandrin B in Shuangwu capsules. MethodsThe HPLC method was employed using a Diamonsil C18 column (200 mm×4.6 mm,5 μm) with a mobile phase of methanol-acetonitrile (1∶1,A) and water (B). The gradient elution program was as follows: 0-5 min,35%-60%A; 5-10 min,60%-70%A; 10-50 min,70%-90%A; 50-90 min,90%A. The flow rate was 1 ml/min. The detection wavelength was set at 220 nm and the temperature was at 35℃.ResultsThe linearity was obtained within the range of 1.28-20.40 μg/ml for syringin (r=0.999 7),6.30-100.80 μg/ml for schisantherin (r=0.999 6),1.20-19.20 μg/ml for deoxyschizandrin (r=0.999 8),and 3.75-60.00 μg/ml for schisandrin B (r=0.999 6). The RSD values of precision were less than 1% for all the four components. The results showed that the samples were stable in the room temperature for at least 24 h. The average recovery rates of syringin,schizandrin,deoxyschizandrin and schisandrin B were 99.47%,102.50%,99.21% and 101.86%,respectively. Conclusion Our method is rapid,easy to perform and accurate; it can be used to control the quality of Shuangwu capsules. |
| Key words: Shuangwu capsules syringin schizandrin deoxyschizandrin schisandrin B high pressure liquid chromatography |
