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  • 周瑞瑞,刘爱军,温志鹏,吴 健,刘建国*,苏定冯*.复方卵清蛋白酶解液对低蛋白血症的影响[J].第二军医大学学报,2010,31(5):494-498    [点击复制]
  • ZHOU Rui-rui, LIU Ai-jun, WEN Zhi-peng, WU Jian, LIU Jian-guo*, SU Ding-feng*.Effect of compound ovalbumin proteolysis liquid on hypoproteinemia in beagle dogs and rats[J].Acad J Sec Mil Med Univ,2010,31(5):494-498   [点击复制]
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复方卵清蛋白酶解液对低蛋白血症的影响
周瑞瑞,刘爱军,温志鹏,吴健,刘建国*,苏定冯*
0
(第二军医大学药学院药理学教研室,上海 200433)
摘要:
目的 探讨复方卵清蛋白酶解液(compound ovalbumin proteolysis liquid, COPL)对犬和大鼠低蛋白血症的治疗作用。方法 实验一,50只毕格犬,雌雄各半,利用胆总管结扎的方法制作胆汁淤积性低蛋白血症的模型;实验二,雄性Sprague-Dawley (SD) 大鼠150只,采用口服苯巴比妥钠、10% 乙醇及腹腔注射四氯化碳(CCl4)方法制作化学性低蛋白血症模型;实验三,雄性SD大鼠150只,手术切除右肾后,采用注射多柔比星(阿霉素)方法 (手术后1周5 mg/kg,手术后4周3 mg/kg)制备肾性低蛋白血症模型。以上模型以血清白蛋白低于30 g/L为成模标准。选取造模成功动物,随机分为5组:模型对照组,COPL低、中、高剂量组以及脱脂奶粉组,处理2周,观察药物对动物血清白蛋白 (serum albumin, SAB)、总蛋白 (total serum protein,TSP)、丙氨酸转氨酶 (alanine transaminase,ALT)、总胆红素 (total bilirubin, TB)、肌酐(creatinine, Cr)、尿素氮 (urinary nitrogen, UN)水平的影响。结果 COPL中、高剂量组对3种模型动物SAB均具有升高作用 (P<0.05或P<0.01),3个剂量组对ALT有降低作用(P<0.05或P<0.01)。各组Cr、UN等指标均没有显著变化。结论 COPL能够升高低蛋白血症血清蛋白的水平,并且对肝功能具有一定的保护作用。
关键词:  复方卵清蛋白酶解液  低蛋白血症  血清白蛋白  丙氨酸转氨酶
DOI:10.3724/SP.J.1008.2010.0494
投稿时间:2009-08-31修订日期:2010-04-06
基金项目:国家科技部重大专项基金 (2009ZX09303-002),国家自然科学基金 (30500614).
Effect of compound ovalbumin proteolysis liquid on hypoproteinemia in beagle dogs and rats
ZHOU Rui-rui, LIU Ai-jun, WEN Zhi-peng, WU Jian, LIU Jian-guo*, SU Ding-feng*
(Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China)
Abstract:
Objective To study the treatment effect of compound ovalbumin proteolysis liquid(COPL) on the hypoproteinemia in dogs and rats. Methods Ligation of common bile duct was performed to induce the cholestasis in 25 female and 25 male beagle dogs. One hundred and fifty male Sprague-Dawley (SD) rats were first given phenobarbital sodium (i.g.) for 1 week, then they were treated with carbon tetrachloride (CCl4, 40%-50%, 1.25 ml/kg, i.p., twice a week) combined with edible alcohol (10%) for 8 weeks. Another 150 male SD rats received exairesis operation of right kidney, and then were treated with adriamycin twice (5 and 3 mg/kg, i.p. on the first and forth week after operation, respectively). The serum levels of serum albumin (SAB), total serum protein (TSP), alanine transaminase(ALT), total bilirubin (TB), creatinine (Cr), and urinary nitrogen (UN) were observed. Animals with SAB level lower than 30 g/L were taken as successful model. The model animals were randomly divided into 5 groups: model control group, 3 COPL treatment groups (low, middle and high dose groups) and non-fat milk group. After treatment for 2 weeks, the animals were killed and the sera were collected for detection of these parameters again. Results The levels of SAB were significantly increased (P<0.05,P<0.01) in meddle and high dose COPL groups and the levels of ALT were significantly decreased (P<0.05,P<0.01) in all the 3 COPL groups in all the 3 hypoproteinemia models. Other indices had no significant changes (P>0.05). Conclusion COPL has satisfactory effect on the hypoproteinemia both in the dogs and rats. Moreover, it has protective effect on the hepatic function.
Key words:  compound ovalbumin proteolysis liquid  hypoproteinemia  serum albumin  alanine transaminase