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| 块花柳珊瑚化学成分的研究 |
| 徐圆缘，李玲，易杨华*，张文* |
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| (第二军医大学药学院海洋药物研究中心，上海 200433) |
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| 摘要: |
| 目的 对采自中国广西省北海附近的块花柳珊瑚(Anthogorgia sp.)进行化学成分的研究。方法 用硅胶柱层析(石油醚∶丙酮=99∶1, 49∶1, 39∶1, 34∶1, 29∶1, 24∶1, 19∶1, 14∶1, 11∶1, 9∶1, 8∶1, 7∶1, 6∶1, 5∶1, 4∶1, 3∶1, 2∶1, 1∶1, 100% 丙酮；氯仿∶甲醇=24∶1, 19∶1, 14∶1, 9∶1, 7∶1, 5∶1, 3∶1, 1∶1, 1∶4, 1∶9,1∶39, 100%甲醇) 梯度洗脱和Sephadex LH-20凝胶柱层析(正己烷∶氯仿∶甲醇2∶1∶1洗脱) 对块花柳珊瑚丙酮提取物的乙醚部分进行分离纯化，并利用经1H NMR、13C NMR、MS等现代光谱技术对其进行结构鉴定。结果 分离得到6个甾醇类化合物以及1个神经酰胺，其结构分别为：麦角甾-5,24(28)-二烯-3β-醇(1)、(22E,24R)-麦角甾-7,22-二烯-3β,5α,6β-三醇(2)、(22E,24S)-麦角甾-5,22-二烯-3β-醇(3)、胆甾-5-烯-3β,7β,19α-三醇(4)、(22E)-胆甾-5,22-二烯-3β-醇(5)、胆甾醇(6)、N-正十六碳酰基-正十八碳-4(Ｅ)-烯鞘胺醇(7)。结论 这些化合物均首次从该种海洋动物中分离得到。 |
| 关键词: 块花柳珊瑚 甾醇类 神经酰胺类 |
| DOI：10.3724/SP.J.1008.2010.0421 |
| 投稿时间：2010-01-18修订日期：2010-03-20 |
| 基金项目:国家高技术研究发展计划重点项目(“863”计划，2006AA09Z446)，上海市重点学科建设项目(B906). |
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| Studies on chemical constituents of Anthogorgia sp. |
| XU Yuan-yuan, LI Ling, YI Yang-hua*, ZHANG Wen* |
| (Research Center for Marine Drugs, School of Pharmacy, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| Objective To study the chemical constituents of Anthogorgia sp. collected from Beihai area of Guangxi Zhuang Autonomous Region, China. Methods The Et2O extract of Anthogorgia sp. was subjected to repeated silica gel (eluted with Pe∶Ac=99∶1, 49∶1, 39∶1, 34∶1, 29∶1, 24∶1, 19∶1, 14∶1, 11∶1, 9∶1, 8∶1, 7∶1, 6∶1, 5∶1, 4∶1, 3∶1, 2∶1, 1∶1, 100%Ac; CHCl3∶MeOH=24∶1, 19∶1, 14∶1, 9∶1, 7∶1, 5∶1, 3∶1, 1∶1, 1∶4, 1∶9, 1∶39, 100% MeOH) and Sephadex LH-20 column chromatography(n-hexane∶CHCl3∶MeOH=2∶1∶1) for isolation and purification. The structures of the resultant compounds were elucidated using spectroscopic analysis(1H NMR, 13C NMR and MS).Results Six steroids and one ceramide were isolated and identified as: Ergost-5,24(28)-dien-3β-ol(1),(22E,24R)-ergost-7,22-dien-3β,5α,6β-triol(2),(22E,24S)-ergost-5,22-dien-3β-ol(3), cholesta-5-en-3β,7β,19α-triol(4),(22E)-cholesta-5,22-dien-3β-ol(5), cholesterol(6), and N-palmitoyloctadecasphinga-4(E)-ene(7). Conclusion All the six compounds are isolated from Anthogorgia sp. for the first time. |
| Key words: Anthogorgia sp. steroids ceramides |
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