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| 含氢保存液降低大鼠供心保存过程中氧化应激和炎性损伤 |
| 孙向东1,陆方林1,崔勇1,孙学军2,,徐志云1* |
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| (第二军医大学长海医院胸心外科,解放军胸心外科研究所,上海 200433;2. Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China;1. 第二军医大学长海医院胸心外科,解放军胸心外科研究所,上海 200433) |
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| 摘要: |
| 目的 观察含氢保存液对大鼠供心保存过程中氧化应激和炎性损伤的影响。方法 32只SD大鼠随机分4组(n=8):对照组(保存液为HTK液),H1组(保存液为含氢浓度约0.2 mmol/L的HTK液),H2组(保存液为含氢浓度约0.4 mmol/L的HTK液),H3组(保存液为含氢浓度约0.8 mmol/L的HTK液)。采用Langendorff离体大鼠心脏灌注法,心脏分别在各组保存液中冷存(4℃) 6 h，复灌后硫代巴比妥酸法检测心肌组织中MDA含量,黄嘌呤氧化酶法检测心肌组织中SOD活力,ELISA法检测心肌组织中8-羟基脱氧鸟苷、TNF-α、IL-6含量。结果 供心冷保存(4℃) 6 h后,H1、H2、 H3组MDA含量低于对照组(P<0.01,P<0.05)。H2、H3组SOD活力高于对照组(P<0.01,P<0.05)；对照组8-羟基脱氧鸟苷水平高于H1、H2、H3组(P<0.01),对照组TNF-α、IL-6含量也高于H1、H2、H3组(P<0.01,P<0.05)。结论 含氢保存液能降低供心保存过程中的心肌氧化损伤,减少炎性细胞因子的产生。 |
| 关键词: 氢 心脏移植 器官保存液 再灌注损伤 氧化性应激 炎性损伤 |
| DOI：10.3724/SP.J.1008.2010.00 |
| 投稿时间：2010-04-28修订日期：2010-06-24 |
| 基金项目: |
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| Hydrogen-containing preservation solution relieves oxidative stress and inflammatory damage of rat donor heart |
| 1. Department of Cardiothoracic Surgery, Institute of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;,XU Zhi-yun1* |
| (2. 第二军医大学海军医学系潜水医学教研室,上海 200433;1. Department of Cardiothoracic Surgery, Institute of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| Objective To study the effect of hydrogen-containing preservation solution against oxidative stress and inflammatory damage of rat donor heart.Methods Thirty-two SD rats were evenly randomized into four groups(n=8): control group (the hearts were protected by HTK solution), H1 group (hydrogen concentration was about 0.2 mmol/L in the HTK solution), H2 group (hydrogen concentration was about 0.4 mmol/L in the HTK solution) and H3 group (hydrogen concentration was about 0.8 mmol/L in the HTK solution). The rat hearts were harvested in all groups and were mounted on the Langendorff apparatus to estimate baseline hemodynamic values. All the hearts underwent hypothermic (4℃) storage for 6 h in the corresponding cardioprotective solutions. Then, superoxide dismutase (SOD) activities and malondiadehyd (MDA) contents in myocardium tissues were measured after reperfusion by xanthine oxidase method and TBA method, respectively. The levels of 8-hydroxydeoxygunosine, TNF-α, and IL-6 in the myocardium tissues were measured by enzyme-linked immunosorbent assay (ELISA). Results The MDA levels in H1, H2, and H3 groups were lower than that in the control group 6 h after preservation (P<0.01 or P<0.05), and the SOD activities in H2, H3 groups were higher than that in the control group (P<0.01 or P<0.05). The levels of 8-hydroxydeoxygunosine, TNF-α, and IL-6 in the control group were higher than those in H1, H2, and H3 groups (P<0.01 or P<0.05). Conclusion Hydrogen-containing preservation solution can relieve the oxidative damage and reduce production of inflammation factors during preservation of rat donor heart. |
| Key words: hydrogen heart transplantation organ preservation solutions reperfusion injury oxidative stress inflammatory damage |
