Objective To establish an HPLC method for determination of baicalin and wogonoside contents in Xiaochaihu decoction. Methods Decoction pieces were mixed and decocted with water. The chromatographic column was Agilent Zorbax XDB-C18(150 mm×4.6 mm, 5 μm); the mobile phase was composed of 0.2% phosphate acid-water(A) and acetonitrile (B) with gradient elution at a flow rate of 1 ml/min. The detection wavelength was set at 275 nm, the temperature of column was 25℃, and the injection volume was 15 μl. Results Baicalin and wogonoside were separated at baseline within 30 min with good linearity; the standard curves for baicalin and wogonoside were Y=44.16X-36.22 (r=0.999,9) and Y=52.08X-28.69(r=0.999,9), respectively. The intra-day and inter-day precisions were both less than 1%, and the limits of qualification were 0.615,6 μg/ml for baicalin and 0.220,8 μg/ml for wogonoside. The recovery rates (n=6) were 95.73% (RSD=0.8%) for baicalin and 97.02% (RSD=1.56%) for wogonoside. Conclusion The method is simple, accurate, stable, and reliable in determining the contents of baicalin and wogonoside in Xiaochaihu decoction, and it can be used for the quality control of this preparation.