| 摘要: |
| 目的预测肿瘤相关蛋白EIF4G1亚型的B细胞表位。方法下载EIF4G1蛋白各种亚型的氨基酸序列，以单参数(亲水性、可及性、柔韧性、抗原性)预测为基础，结合ABCpred和二级结构预测筛选等方法综合筛选EIF4G1蛋白质亚型特异性的B细胞表位。结果目前已知EIF4G1蛋白的亚型有5种，亚型间的氨基酸变化局限在一个由300个氨基酸形成的序列区域，亚型特异性的B细胞表位可能位于该区域的14~19、21~27、52~61、106~112、113~139、183~189、201~216和217~224位氨基酸残基区域内或附近。结论EIF4G1蛋白质亚型间存在特异性B细胞表位，这些表位对应用合成肽抗原检测蛋白质亚型和进行肿瘤患者的早期诊断具有重要指导意义。 |
| 关键词: 真核细胞起始因子4G 蛋白质亚型 B淋巴细胞表位 肿瘤 |
| DOI：10.3724/SP.J.1008.2010.0822 |
| 投稿时间：2010-05-10修订日期：2010-06-21 |
| 基金项目:国家自然科学基金(30872562), 上海市教育委员会科研创新项目(08ZZ39). |
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| Prediction of B cell epitopes of tumor-associated protein EIF4G1 subtypes |
| CAO Ting-hu1, CHANG Wen-jun2, HU Jin-xin1, ZHU Jun1, CAO Guang-wen2*,CAO Guang-wen2* |
| (1. Department of Urology, Shanghai Baoshan Central Hospital, Shanghai 201900, China;;2. Department of Epidemiology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| ObjectiveTo predict the B cell epitopes of tumor-associated protein EIF4G1 subtypes.MethodsThe sequences of all the protein subtypes of EIF4G1 were retrieved from NCBI protein database.Based on single parameter evaluation,including hydrophilicity,flexility,antigenicity,the B cell epitopes of the EIF4G1 protein subtypes were predicted using NPS@structure software and ABCpred software.ResultsEIF4G1 protein had five subtypes.The variation of the five different EIF4G1 subtypes was limited within a 300aa region.We identified eight epitopes locating in or near 14-19,21-27,52-61,106-112,113-139,183-189,201-216, and 217-224, which can be used to identify specific B cell epitopes of different protein subtypes.ConclusionB cell epitopes of EIF4G1 protein subtypes do exist,and they may be used for the protein subtypes evaluation and early diagnosis of tumor patients using artificially-produced matched peptides. |
| Key words: eukaryotic initiation factor-4G protein isofroms B lymphocytes epitopes |
