| 摘要: |
| 目的研究5-脂氧合酶(5-LOX)和核因子κB P65(NF-κB P65)在溃疡性结肠炎(UC)患者结肠黏膜的表达,并探讨二者的关系。方法取32例UC患者结肠黏膜标本进行组织学分级,同时收集正常对照者结肠黏膜标本26例。分别采用实时荧光定量RT-PCR和免疫组织化学法检测UC患者结肠黏膜5-LOX、NF-κB P65的表达,并分析其相互关系。结果32例UC患者组织学分级1 级11 例,2 级17 例,3 级4 例。UC患者结肠黏膜5-LOX、NF-κB P65表达高于正常对照组,差异有统计学意义(P<0.05),二者的阳性表达率随UC组织学分级增加而增加；5-LOX与NF-κB P65呈正相关。结论5-LOX和NF-κB P65在UC结肠炎症发生发展中可能发挥重要作用。 |
| 关键词: 溃疡性结肠炎 NF-κB 5-脂氧合酶 |
| DOI：10.3724/SP.J.1008.2011.0339 |
| 投稿时间：2010-09-24修订日期：2011-01-26 |
| 基金项目:海南省卫生厅资助课题(琼卫2009-8). |
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| Expression of 5-LOX and NF-κB P65 in mucosal tissues of patients with ulcerative colitis and their relationship |
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| Abstract: |
| [Abstract]Objective: To investigate and significance the expression of 5-LOX(5-lipoxygenase), NF-kB P65( Nuclear factor kappa B P65 )in colon mucosa of ulcerative colitis(UC)and to analyze their correlation．Methods: The biopsies from 32 UC patients and 26 healthy controls were collected，and the expression of 5-LOX, NF-kB P65 mRNA and protein in colonic mucosa was determined by Real-time fluorescent quantitative reverse transcription polymerase chain reaction(RT-PCR) and immunohistochemistry assay respectively．The patients were graded according to endoscopic and histological grading．Results: The expressions of 5-LOX, NF-kB P65 mRNA and protein in UC patients were significantly increased than those in healthy controls, which wag correlated with histological grading. A good positive correlation was found between 5-LOX and NF-kB P65.Conclusion: The expression of 5-LOX is closely related to NF-kB P65 in colon mucosa from UC patients. They may play a pivotal role in inflammation of UC. |
| Key words: Colitis,ulcerative 5-lipoxygenase Nuclear factor kappa B Real-time fluorescent quantitative reverse transcription polymerase chain reaction Immunohistochemistry assay |
