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  • 李叶晟1△,徐峰1△,李洪海2,吕欣3,杨甲梅1*.电针刺激大鼠足三里穴对肝缺血再灌注损伤的保护作用[J].第二军医大学学报,2011,32(2):191-194    [点击复制]
  • LI Ye-sheng1△, XU Feng1△, LI Hong-hai2, L Xin3, YANG Jia-mei1*.Electroacupuncture of Zusanli acupuncture point protects against hepatic ischemia/reperfusion injury in rats[J].Acad J Sec Mil Med Univ,2011,32(2):191-194   [点击复制]
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电针刺激大鼠足三里穴对肝缺血再灌注损伤的保护作用
李叶晟1△,徐峰1△,李洪海2,吕欣3,杨甲梅1*
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(1.第二军医大学东方肝胆外科医院特需治疗科,上海200438; 2.上海市肿瘤研究所,上海200240; 3.上海市肺科医院麻醉科,上海200433)
摘要:
\[摘要\]目的探讨电针刺激大鼠足三里穴对肝缺血再灌注损伤的保护作用及可能机制。方法40只雄性SD大鼠被随机分为假手术(A)组、肝缺血再灌注(B)组、非经络穴位电针刺激(C)组和足三里穴电针刺激(D)组,每组10只。B组不给予电针刺激;C、D两组于缺血前分别针刺非经络穴位和足三里穴,强度均为4 mA、2/100 Hz的电刺激,持续45 min。B、C、D组以3 cm血管阻断夹阻断左横叶及中叶的入肝血流,保留右叶及尾叶血供,造成70%的肝组织缺血,90 min后恢复血供,再灌注后8 h从下腔静脉抽血,采用全自动生化分析仪测定血清丙氨酸转氨酶(ALT);同时处死大鼠,以实时定量PCR法检测肝组织肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)等细胞因子mRNA的表达。结果B、C、D组与A组比较,ALT、TNF-α和IL-10均有显著升高,差异具有统计学意义(P<0.05),D组ALT和TNF-α升高幅度均显著低于B和C组(P<0.05);而B、C、D组间IL-10差异无统计学意义(P>0.05)。结论电针刺激足三里穴能有效减轻肝脏缺血再灌注损伤,其作用机制可能与胆碱能抗炎通路有关。
关键词:  电针  足三里穴    再灌注损伤
DOI:10.3724/SP.J.1008.2011.0191
投稿时间:2010-11-29修订日期:2011-01-18
基金项目:
Electroacupuncture of Zusanli acupuncture point protects against hepatic ischemia/reperfusion injury in rats
LI Ye-sheng1△, XU Feng1△, LI Hong-hai2, L Xin3, YANG Jia-mei1*
(1. Special Treatment Department, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China; 2. Shanghai Cancer Institute, Shanghai 200240, China; 3. Department of Anesthesiology, Shanghai Pulmonary Hospital, Tongji University, Shanghai 200433, China)
Abstract:
\[Abstract\]ObjectiveTo study the protective effects of electroacupuncture of Zusanli acupuncture on rat hepatic ischemia/reperfusion injury and the possible mechanism. MethodsForty male SD rats were randomly divided into 4 groups (each group n=10):sham(A), ischemia/reperfusion(B), ischemia/reperfusion + non-electroacupunture(C), and ischemia/reperfusion + electroacupuncture of Zusanli acupuncture point(D). Animals in C and D groups received electroacupuncture of Zusanli acupuncture point (4 mA, 2/100 Hz) before ischemia for 45 min. Hepatic ischemia/reperfusion injury (70%) was induced by blocking blood inflow of the left transverse lobe and middle lobe for 90 min, then reperfusion was allowed for 8 h; blood samples were obtained from the inferior vena cava for determination of serum ALT. Meanwhile, real-time PCR was used to examine the heptaic expression of TNF-α and IL-10. ResultsB, C, and D groups demonstrated significantly higher levels of serum ALT and hepatic TNF-α and IL-10 compared with A group(P<0.05), and the increase of ALT and TNF-α in D group was significantly lower than those in B and C group (P<0.05). Expression of IL-10 was not significantly different between the 3 groups (P>0.05). ConclusionElectroacupuncture of Zusanli acupuncture point can effectively protect against hepatic ischemia/reperfusion injury, which is possibly related to the cholinergic anti-inflammatory pathway.
Key words:  electroacupuncture  point ST36(Zusanli)  liver  reperfusion injury