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  • 杨丰1,2 △,曹永兵2 △,颜天华1,王秋娟1,季晖1*,姜远英2*.长期高盐刺激诱导白假丝酵母菌耐受氧化应激[J].第二军医大学学报,2011,32(2):139-143    [点击复制]
  • YANG Feng1,2 △, CAO Yong-bing2 △, YAN Tian-hua1, WANG Qiu-juan1, JI Hui1*, JIANG Yuan-ying2*.Long-term exposure to high concentration of salt stress induces antioxidative response in Candida albicans[J].Acad J Sec Mil Med Univ,2011,32(2):139-143   [点击复制]
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长期高盐刺激诱导白假丝酵母菌耐受氧化应激
杨丰1,2△,曹永兵2△,颜天华1,王秋娟1,季晖1*,姜远英2*
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(1. 中国药科大学药学院生理学教研室,南京 210009; 2. 第二军医大学药学院新药研究中心,上海 200433)
摘要:
\[摘要\]目的观察长期高浓度氯化钠刺激对白假丝酵母菌耐受氧化应激能力的影响。方法选用2株白假丝酵母菌国际标准株SC5314和ATCC76615,用含有1 mol/L氯化钠的YEPD培养液连续培养24 d,考察子代与亲本菌对氧化应激的耐受能力。采用real-time RT-PCR考察白假丝酵母菌抗氧化酶的转录水平,并测定其抗氧化酶的活性,利用丙二醛(MDA)测定试剂盒衡量白假丝酵母菌脂质过氧化损伤,并测量白假丝酵母菌菌体细胞内活性氧水平。结果长期用高浓度氯化钠刺激能增加白假丝酵母菌对过氧化氢(12 mmol/L)和咪康唑(4 μg/ml)的耐受性,促进其抗氧化酶的转录,使抗氧化酶SOD1、CAT1、谷胱甘肽还原酶(GR,转录基因为TTR1 )的转录水平升高2~5倍,增强抗氧化酶的活性(P<0.05),降低脂质过氧化损伤(P<0.01),减少菌体细胞内活性氧水平(P<0.001)。结论长期高浓度氯化钠刺激能够增强白假丝酵母菌耐受氧化应激的能力。
关键词:  白假丝酵母菌  氯化钠  氧化性应激  抗氧化酶  脂质过氧化作用  活性氧
DOI:10.3724/SP.J.1008.2011.0139
投稿时间:2010-12-09修订日期:2011-01-11
基金项目:国家高技术研究发展计划(2008AA02Z128), 国家重点基础研究发展计划(2005CB523105), 上海市基础研究重点项目(08JC1405900).
Long-term exposure to high concentration of salt stress induces antioxidative response in Candida albicans
YANG Feng1,2 △, CAO Yong-bing2 △, YAN Tian-hua1, WANG Qiu-juan1, JI Hui1*, JIANG Yuan-ying2*
(1. Department of Physiology, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, Jiangsu, China; 2. Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China)
Abstract:
\[Abstract\]ObjectiveTo investigate the effect of long-term exposure to high concentration of sodium chloride on resistance to oxidative stress in Candida albicans (C. albicans). MethodsTwo C. albicans strains, SC5314 and ATCC76615, were challenged with 1 mol/L NaCl for 24 consecutive days. Spot assay was performed to compare the resistance to hydrogen peroxide and miconazole between the wild-type and derivative strains. The transcription levels of antioxidant enzymes, including superoxide dismutase (SOD1), catalase (CAT1), and glutathione reductase (GR), were determined by real-time RT-PCR, and their activities were also examined. Peroxidation of lipid was examined by determining the contents of malondialdehyde (MDA); the cellular level of reactive oxygen species (ROS) was also determined.ResultsLong-term exposure to high concentration of sodium chloride enhanced the resistance of C. albicans to oxidative stress (hydrogen peroxide \[12 mmol/L\] and miconazole \[4 μg/ml\]); the exposure increased the transcription of antioxidant enzymes, SOD1, CAT1, and GR by 2-5 folds; and it also significantly increased the activities of the antioxidant enzymes (P<0.05). Meanwhile, it significantly alleviated the peroxidation of lipid (P<0.01) and decreased the intracellular ROS contents (P<0.01). ConclusionLong-term exposure to salt stress can increase the resistance to oxidative stress in C. albicans.
Key words:  Candida albicans  sodium chloride  oxidative stress  antioxidant enzymes  lipid peroxidatation  reactive oxygen species