| 摘要: |
| 目的探讨基于TEM8的腺病毒(Ad-TEM8)对小鼠胶质瘤G422细胞系体内外模型的生长抑制及其抗血管的生成作用。方法连续皮下注射Ad-TEM8使其激活小鼠免疫系统并收集小鼠脾淋巴细胞,将该细胞与G422细胞体外共培养,检测G422细胞的生长抑制率,并应用ELISOT检测该免疫激活淋巴细胞在体外分泌IFN-γ的能力。另构建皮下荷G422昆明小鼠在体肿瘤模型,并连续皮下注射Ad-TEM8后检测肿瘤生长速度以及瘤内血管抑制效应。 结果Ad-TEM8能有效激发小鼠产生具有肿瘤生长抑制作用的毒性效应淋巴细胞,并分 |
| 关键词: 神经胶质瘤；TEM8 腺病毒； G422细胞 病理性新生血管化 |
| DOI：10.3724/SP.J.1008.2011.0860 |
| 投稿时间：2010-12-16修订日期：2011-03-15 |
| 基金项目: |
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| Inhibitory effect of recombinant adenovirus expressing TEM8 gene against angiogenesis in mouse model of glioma |
| CHEN Hong-jie1,2,WANG Shou-sen1,ZHENG Zhao-cong1,WANG Ru-min1 |
| (1. Department of Neurosurgery, Fuzhou General Hospital, PLA Nanjing Military Area Command, Fuzhou 350025, Fujian, China;2. PLA Fuzhou General Hospital, Clinical College of Fujian Medical University, Fuzhou 350025, Fujian, China) |
| Abstract: |
| ObjectiveTo evaluate the inhibitory effects of recombinant adenovirus carrying TEM8 (Ad-TEM8) against angiogenesis and growth of mouse glioma G422 cells in vitro and in vivo. MethodsThe Ad-TEM8 was constructed and subcutaneously injected to immunize Kunmi |
| Key words: glioma TEM8 adenovirus G422 cells pathologic neovascularization |
