| 摘要: |
| 目的探讨曲古抑菌素A(TSA)和丁酸钠(NaB)对人外周血CD4+T细胞向Th1/Th2分化的影响。方法体外分离并培养人外周血CD4+T细胞,分为TSA组(0.33 μmol/L)、NaB组(1.4 mmol/L)和对照组(CON组)。采用MTT、流式细胞术、qRT-PCR和酶联免疫吸附试验(ELISA)法检测TSA组、NaB组及对照组细胞因子IFN-γ、IL-4表达水平及IFN-γ+细胞/IL-4+细胞比值。结果外周血CD4+T细胞在植物血凝素(PHA)刺激48 h后,ELISA检测细胞培养上清显示:TSA组、NaB组上清IFN-γ、IL-4分泌水平均明显高于对照组(P<0.05)。qRT-PCR检测显示:TSA组、NaB组IFN-γ mRNA表达高于对照组,相比对照组提高到(3.12±0.34)、(2.3±0.51)倍,差异有统计学意义(P<0.05);IL-4 mRNA提高到(1.87±0.42)、(1.63±0.51)倍,差异有统计学意义(P<0.01)。流式细胞术对胞内因子检测显示:TSA组、NaB组IFN-γ+细胞/IL-4+细胞比值明显升高,是对照组的1.93、1.55倍,差异有统计学意义(P<0.01)。结论TSA、NaB暴露可引起人外周血CD4+T细胞Th1/Th2分化平衡改变,出现向Th1方向的“Th1/Th2平衡的漂移”。 |
| 关键词: 曲古抑菌素A 丁酸钠 辅助T细胞 干扰素γ 白介素4 |
| DOI：10.3724/SP.J.1008.2011.0621 |
| 投稿时间：2011-02-22修订日期：2011-04-19 |
| 基金项目:江苏省高校自然科学基金(10KJB310010),江苏省高校优势学科建设工程资助项目. |
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| Effect of trichostatin A and sodium butyrate on variation of Th1/Th2 cell balance after differentiation of peripheral blood CD4+T cells |
| ZHANG Jie,L Li-jun,ZHOU Ming-ming,SUN Xiao-lei,WANG Xiao-ying* |
| (Department of Immunology, College of Medicine, Nantong University, Nantong 226019, Jiangsu, China) |
| Abstract: |
| ObjectiveTo investigate the effects of trichostatin A(TSA) and sodium butyrate(NaB), histone deacetylase inhibitors, on the balance of Th1/Th2 after differentiation of peripheral blood CD4+T cells. MethodsPeripheral CD4+T cells were grown in vitro and were divided into TSA group, NaB group, and CON group. The concentrations of IL-4 and IFN-γ (Th1/Th2 factors) in the supernatants were examined by ELISA in each group 48 h later; the expressions of IL-4 and IFN-γ were analyzed by qRT-PCR; and the T-lymphocytes in peripheral blood (PBL) were measured by flow cytometry. Results ELISA showed that the supernatant IFN-γ and IL-4 levels in the TSA and NaB groups were significantly higher than those in the CON group 48 h after treatment with TSA(0.33 μmol/L) and NaB(1.4 mmol/L); real-time quantitative PCR also showed that the expressions of IFN-γ and IL-4 mRNA in TSA and NaB groups were significantly higher than those in the CON group(3.121, 2.31; P<0.05). However, flow cytometry showed that the ratios of IFN-γ+ cells/IL-4+ cells were significantly higher in TSA and NaB groups than that in the CON group((1.931,1.551;P<0.05) .ConclusionTreatment with TSA and NaB, both histone deacetylase inhibitors,can induce a shift of Th1/ Th2 cell balance toward Th1 cells in peripheral CD4+T cells. |
| Key words: trichostatin A sodium butyrate Th1 cells Th2 cells interferon-γ interleukin-4 |
