ObjectiveTo observe the effect of microRNA-29c on the proliferation and invasion abilities of human prostate cancer cell line LNCaP, and to discuss its potential application. MethodsThe difference in miR-29c expression between ADPC (androgen-dependent prostate cancer) and AIPC (androgen-independent prostate cancer) cell lines was observed by real time RT-PCR．MiR-29c-inhibitor(anti-miR-29c)was used to decrease miR-29c expression in LNCaP cells; then the cell proliferation was examined with CCK-8 assay, the cell cycle was analyzed using flow cytometry, and the invasive abilities of LNCaP cells were observed by transwell invasion assay before and after treatment with anti-miR-29c. ResultsThe result of real-time RT-PCR showed that miR-29c expression in LNCaP-AI cells was significantly lower than that in LNCaP cells (P<0.05). Down-expression of miR-29c in LNCaP cells significantly promoted the proliferation and invasion abilities of LNCaP cells (P<0.05). ConclusionNormal expression of miR-29c plays an inhibitory role in the proliferation and invasion of LNCaP cells, indicating it might be a new target for biotherapy of prostate cancer.