Thromboplastin reagents were made from natural tissues of human or animals before the emergence of tissue factor (TF) which can be purified in 1990s. In recent years, highly purified recombinant tissue factor (rTF) or soluble tissue factor (sTF) has been used to prepare thromboplastin reagents. This article reviewed the evolution of technique for preparing recombinant tissue factor-based thromboplastin reagents both at home and abroad, and summarized the advantages of recombinant tissue factor and analyzed the future of thromboplastin reagents. It has been pointed out that highly sensitive and stable thromboplastin reagents are needed in the future.