Objective To conduct item analysis on MMPI Prejudice Scale(Pr) and to develop a simplified version of the scale. Methods Pr and Symptom Checklist 90(SCL-90) were used to examine 3,767 newly recruited soldiers. The difficulty degree and discrimination degree of each item of Pr were analyzed; the correlation between each item score and total score was analyzed; and a simplified version of Pr was developed based on the results of item analysis. The Cronbach’s α coefficient values of the original scale and the simplified scale were calculated, and the criterion validity was analyzed using SCL-90 as the validity criterion. For other samples, 16PF, EPQ, and SAS were tested and the simplified scale was also tested at the same time. Results The difficulty degrees of the original scale ranged 0.04-0.69, with those of 17 items being less than 0.20. The discrimination degree ranged 0.10-0.72, with those of 12 items being less than 0.30. The correlation coefficient of the item score and the total score ranged 0.19-0.60, with those of 9 items being less than 0.30. The simplified scale composed of 20 items with better quality. The Cronbach’s α coefficient values of the original scale and the short-versioned scale were 0.801 and 0.757, respectively. The total scores of both the original scale and the short-versioned scale were significantly correlated with the scores of SCL-90, with the correlation coefficient being above 0.38. The correlation coefficient between the score of the simplified scale and that of the original scale was 0.967. The correlation coefficient between the score of the simplified scale and those of 16PF, EPQ, SAS was significant. Conclusion The simplified scale in this study has a higher reliability and validity.