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  • 金以超,邱啸臣,孙瑜,罗鹏飞,李武全,付晋凤,夏照帆.富氢林格液减轻严重烧伤大鼠延迟复苏引起的肠损伤[J].第二军医大学学报,2012,33(2):170-174    [点击复制]
  • JIN Yi-chao,QIU Xiao-chen,SUN Yu,LUO Peng-fei,LI Wu-quan,FU Jin-feng,XIA Zhao-fan.Protective effect of hydrogen-lactated Ringer’s solution against extensive burn-induced intestine injury in rats after delayed fluid resuscitation[J].Acad J Sec Mil Med Univ,2012,33(2):170-174   [点击复制]
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富氢林格液减轻严重烧伤大鼠延迟复苏引起的肠损伤
金以超1,2,邱啸臣1,孙瑜1,罗鹏飞1,李武全1,2,付晋凤2,夏照帆1*
0
(1. 第二军医大学长海医院烧伤科,上海 200433
2. 昆明医学院第二附属医院烧伤科,云南省烧伤研究所,昆明 650101
*通信作者)
摘要:
目的 观察富氢林格液(富氢液)对延迟复苏严重烧伤大鼠肠组织的保护作用。方法 制备富氢液,采用随机数字表法将36只SD大鼠随机分为3组(每组12只),即假烫组、烫伤+普通林格液组和烫伤+富氢液组,在后两组大鼠背部造成占体表面积(TBSA) 30% 的Ⅲ度烫伤,并于烫伤后7 h、9 h、17 h,分别经腹腔补给相当于总补液体积1/2、1/4、1/4的普通林格液或富氢液,假烫组不补液。24 h后取小肠组织做H-E染色,观察病理变化,并检测肠组织中丙二醛(MDA)含量和髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)活性,以及促炎细胞因子IL-1β 和 TNF-α的含量。结果 相对于普通林格液组,富氢液组小肠黏膜损伤减轻,肠组织MDA含量(P<0.05)及MPO和SOD活性均降低(P<0.01);促炎细胞因子IL-1β 和 TNF-α含量在富氢液组均显著低于普通林格液组(P<0.01)。结论 富氢液对烧伤延迟复苏后肠组织具有保护作用,可能主要从两方面体现:一是降低大面积烧伤及延迟复苏所带来的氧化损伤;二是抑制延迟复苏大鼠肠组织促炎因子IL-1β和TNF-α的生成。
关键词:  烧伤  小肠  延迟复苏    炎症因子  脂质过氧化作用
DOI:10.3724/SP.J.1008.2012.0170
投稿时间:2011-08-16修订日期:2011-12-22
基金项目:
Protective effect of hydrogen-lactated Ringer’s solution against extensive burn-induced intestine injury in rats after delayed fluid resuscitation
JIN Yi-chao1,2,QIU Xiao-chen1,SUN Yu1,LUO Peng-fei1,LI Wu-quan1,2,FU Jin-feng2,XIA Zhao-fan1*
(1. Department of Burns, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
2. Department of Burns, Burn Institute of Yunnan Province, Second Affiliated Hospital of Kunming Medical College, Kunming 650101, Yunnan, China
*Corresponding author.)
Abstract:
Objective To investigate the protective effect of hydrogen-rich lactated Ringer’s solution (HRS) against intestine injury induced by extensive burn in Sprague-Dewley (SD) rat model after delayed fluid resuscitation. MethodsThirty-six male SD rats were randomly divided into three groups (n=12): sham-burn group(S group), burn plus normal lactated Ringer’s solution(NRS) group (BR group) and burn plus hydrogen-rich lactated Ringer’s solution(HRS) group (BH group). Rats in the S group were immersed into 37℃ water without fluid replacement; those in BR group and BH group were subjected to 30% total body surface area (TBSA) Ⅲ degree full-thickness scald. At first, a total of 2 ml·(1% TBSA)-1·kg-1 of HRS or NRS were replaced at 7 h post-burn and half volume of the total solution was replaced at the 9 h and 17 h. All rats were sacrificed at 24 h. Small intestine tissues were removed for H-E staining and determination of MDA content, MPO and SOD activity; and the levels of the IL-1β and TNF-α were determined by ELISA. ResultsThe intestinal injury was relieved in BH group compared with BR group. The intestinal MDA content (P<0.05) and activities of MPO and SOD (P<0.01) were all decreased in BH group. IL-1β and TNF-α levels in BH group were significantly lower than those in the BR group (P<0.01). ConclusionHRS can attenuate intestine injury induced by extensive burn after delayed fluid resuscitation. It can decrease the oxidative injury following extensive burn and delay fluid resuscitation, and it can also suppress the generation of proinflammatory cytokine IL-1β and TNF-α.
Key words:  burns  small intestine  delayed resuscitation  hydrogen  inflammatory factors  lipid peroxidation