The high resolution magic angle spinning NMR spectroscopy (HRMAS) can be directly used to examine in vitro tissues, needing no extracting and separating procedures. Moreover, it can enhance signal intensity and improve resolution of images. HRMAS can directly obtain the structural and conformational information (molecular level) of in vitro tumor samples of the brain, liver, kidney, and lung, which gives it a bright future in early diagnosis of tumors. The present paper mainly reviews the applications of HRMAS in tumor metabonomics research