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| S100A7表达下调对胃癌SGC-7901细胞增殖和迁移能力的影响 |
| 赵洁1,王薇1,孙红英1,陈会军1,毛海州1,曾燕1,盛之玲2* |
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(1.解放军155中心医院消化内科,开封 475003
2.郑州大学基础医学院生理学教研室,郑州 450052
*通信作者) |
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| 摘要: |
| 目的研究S100A7表达下调对胃癌SGC-7901细胞增殖和迁移的影响,并探讨其可能的分子机制。方法将胃癌SGC-7901细胞分为3组: 未转染组、对照siRNA组和S100A7 siRNA组，后两组分别转染对照siRNA和S100A7 siRNA。利用实时荧光定量RT-PCR和蛋白质印迹分析检测各组胃癌SGC-7901细胞中S100A7 mRNA和蛋白的表达；采用CCK-8和Boyden小室分别检测各组细胞增殖和细胞迁移能力的变化。最后采用蛋白质印迹分析法检测3组胃癌细胞中cyclin D1、Cdk2和MMP-2蛋白的表达。结果S100A7 siRNA能下调胃癌SGC-7901细胞中S100A7 mRNA和蛋白的表达。S100A7 mRNA和蛋白表达下调抑制胃癌SGC-7901细胞的增殖和迁移,并降低cyclin D1、Cdk2和MMP-2蛋白的表达。结论S100A7表达下调介导的胃癌细胞增殖抑制和迁移降低可能与cyclin D1、Cdk2和MMP-2表达的下调密切相关。 |
| 关键词: 胃肿瘤 S100A7 小分子干扰RNA 细胞增殖 细胞迁移 |
| DOI：10.3724/SP.J.1008.2012.00851 |
| 投稿时间：2012-04-24修订日期：2012-07-20 |
| 基金项目: |
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| Effect of down-regulated S100A7 expression on proliferation and migration of gastric carcinoma cell line SGC-7901 |
| ZHAO Jie1,WANG Wei1,SUN Hong-ying1,CHEN Hui-jun1,MAO Hai-zhou1,ZENG Yan1,SHENG Zhi-ling2* |
(1. Department of Gastroenterology, No. 155 Hospital of PLA, Kaifeng 475003, Henan, China
2. Department of Physiology, College of Basic Medicine, Zhengzhou University, Zhengzhou 450052, Henan, China
*Corresponding author.) |
| Abstract: |
| ObjectiveTo investigate the effect of down-regulated S100A7 expression on proliferation and migration of gastric carcinoma SGC-7901 cells, and to explore the possible molecular mechanisms. MethodsThe experiment was divided into three groups, including untransfected group, control siRNA group and S100A7 siRNA group. S100A7 siRNA and control siRNA were transfected into gastric carcinoma SGC-7901 cells in S100A7 siRNA group and control siRNA group, respectively. Expressions of S100A7 mRNA and protein in gastric carcinoma SGC-7901 cells were examined by Real-time PCR and Western blotting analysis, respectively. CCK-8 kit and Boyden chamber were used to observe the proliferation and migration of SGC-7901 cells in the three groups. The expressions of cyclin D1, Cdk2 and MMP-2 proteins were also examined by Western blotting analysis in the three groups. ResultsS100A7 siRNA effectively down-regulated the expressions of S100A7 mRNA and protein in gastric carcinoma SGC-7901 cells. Down-regulated S100A7 expression greatly inhibited the proliferation and migration of gastric carcinoma SGC-7901 cells, and also down-regulated expression of cyclin D1, Cdk2 and MMP-2 proteins. ConclusionDown-regulated S100A7 expression-mediated inhibition of gastric carcinoma cell proliferation and migration may be closely associated with the decreased expressions of cyclin D1, Cdk2 and MMP-2 proteins. |
| Key words: stomach neoplasms S100A7 small interfering RNA cell proliferation cell migration |
