| 摘要: |
| 目的 观察A型肉毒毒素(botulinum toxin type A,BTX-A)对P物质(substance P,SP)所致肌条收缩的影响,探讨BTX-A在SP与NK1受体结合过程中可能存在的作用机制。方法 取大鼠胃体、胃底平滑肌制备肌条并随机分为对照组、SP组、SP+APTL-SP(NK1受体拮抗剂)组、BTX-A组、BTX-A+SP组、SP+BTX-A组,采用Biolap420E生物机能实验系统记录肌条收缩数据。结果 SP增加胃体平滑肌自发性收缩张力及振幅、胃底平滑肌自发性收缩张力(P均<0.01)；APTL-SP降低SP引发的胃体、胃底平滑肌收缩张力(P<0.01)；BTX-A作用后的胃体、胃底平滑肌条振幅降低(P均<0.01)。BTX-A降低SP引发的胃体(P<0.05,P<0.01)、胃底(P均<0.01)平滑肌自发性收缩张力及振幅;SP对BTX-A作用后的胃体、胃底平滑肌收缩能力未产生增强作用。结论 SP可增强胃体、胃底平滑肌收缩能力，而BTX-A可抑制SP对胃体、胃底平滑肌的收缩作用。 |
| 关键词: A型肉毒毒素 P物质 胃体 胃底 平滑肌 |
| DOI：10.3724/SP.J.1008.2012.001074 |
| 投稿时间：2012-05-11修订日期：2012-07-05 |
| 基金项目:河南省教育厅自然科学研究项目(2011C310012), 兰州大学医学基金资助项目(LZUYX200605). |
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| Inhibitory effect of botulinum toxin type A on SP-induced rat smooth muscle contractility of gastric body and gastric fundus in vitro |
| ZHOU Yuan-yuan1,2,LI Chao-yan1,HOU Yi-ping2* |
(1. Department of Basic Medicine, Luohe Medical College, Luohe 462002, Henan, China
2. Laboratory of Neurobiology, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, Gansu, China
*Corresponding author.) |
| Abstract: |
| ObjectiveTo observe the effect of botulinum toxin type A (BTX-A) on the SP-induced smooth muscle contractility of gastric body and gastric fundus, so as to investigate the role of BTX-A in the binding between SP and NK1 receptor. MethodsMuscle strips were prepared from gastric body and gastric fundus and were randomly divided into control group, SP group, SP+APTL-SP (NK1 receptor antagonist) group, BTX-A group, BTX-A+SP group, and SP+BTX-A group. The contractility data were recorded by physiological experimental system of Biolap420E. ResultsSP significantly enhanced the tension and amplitude of gastric body contractility and the tension of gastric fundus contractility (P<0.01). APTL-SP signficantly inhibited SP-induced smooth muscle contractility tension in gastric body and gastric fundus (P<0.01). BTX-A significantly inhibited the smooth muscle contractility amplitude in gastric body and gastric fundus (P<0.01). BTX-A significantly inhibited SP-induced smooth muscle contractility, including the tension (P<0.05, 0.01) and amplitude (P<0.01) in the gastric body and gastric fundus. After BTX-A treatment, SP did not enhance the smooth muscle contractility of the gastic body and gastric fundus in vitro. ConclusionSP can enhance the spontaneous contractility of smooth muscle in the gastric body and gastric fundus; BTX-A can inhibit SP-induced smooth muscle contractility of both gastric body and gastric fundus. |
| Key words: botulinum toxin A substance P gastric body gastric fundus smooth muscle |
