| 摘要: |
| 目的观察低氧环境对大鼠大脑皮质细胞分泌IL-1β、IL-6和TNF-α的影响。方法原代培养新生SD大鼠大脑皮质细胞,设1%、4%两个低氧浓度和3 h、6 h两个低氧处理时间,处理结束后采用 ELISA方法检测各组细胞培养液中IL-1β、IL-6和TNF-α的含量。结果与相应的常氧(20%氧)对照组相比,1%氧浓度环境处理细胞3 h和6 h,IL-6含量均下降(P<0.01，P<0.05),IL-1β和TNF-α含量变化无统计学意义(P>0.05)。与相应的常氧(20%氧)对照组相比,4%氧浓度环境处理细胞3 h,IL-1β、IL-6和TNF-α含量变化均无统计学意义(P>0.05)；处理细胞6 h时,IL-1β(P<0.01)和IL-6(P<0.05)均显著下降,TNF-α含量变化无统计学意义(P>0.05)。结论1%氧浓度环境虽对大脑皮质细胞分泌IL-1β和TNF-α无影响,但可抑制细胞分泌IL-6。4%氧浓度环境虽对细胞分泌TNF-α无影响,但对细胞分泌IL-1β和IL-6具有抑制作用。 |
| 关键词: 细胞低氧 大脑皮质 白介素1β 白介素6 肿瘤坏死因子α |
| DOI：10.3724/SP.J.1008.2012.001356 |
| 投稿时间：2012-09-11修订日期：2012-10-25 |
| 基金项目:国家自然科学基金(30870349). |
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| Effects of hypoxia on secretion of IL-1β, IL-6 and TNF-α in cerebral cortex cells of rats |
| LI Hua-hua,LOU Shu-jie* |
(Department of Exercise Biochemistry and Nutrition, School of Kinesiology, Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai 200438, China
*Corresponding author.) |
| Abstract: |
| ObjectiveTo investigate the effects of different hypoxia treatments on secretion of IL-1β, IL-6 and TNF-α in the cerebral cortex cells of rats. MethodsPrimary cultured cerebral cortex cells were subjected to hypoxia treatments (1% and 4% oxygen) for 3 h or 6 h. Enzyme-linked immunosorbent assay (ELISA) was used to determine the levels of IL-1β, IL-6 and TNF-α protein in cultural supernatants. Results(1) Compared with the corresponding normoxia (20% oxygen) controls, IL-6 level was significantly decreased after treatment with 1% oxygen for 3 h and 6 h (P<0.01, P<0.05), and the levels of IL-1β and TNF-α were not significantly different. (2) Compared with the corresponding normoxia (20% oxygen) controls, the levels of IL-1β, IL-6 and TNF-α were not significantly different in cells treated with 4% oxygen for 3 h(P>0.05); the levels of IL-1β and IL-6 were significantly decreased in cells treated with 4% oxygen for 6 h (P<0.01, P<0.05), and TNF-α level was not significantly different(P>0.05). ConclusionOxygen at 1% has no influence on IL-1β or TNF-α secretion in cerebral cortex cells of rats, and it can inhibit the production of IL-6. Oxygen at 4% has inhibitory effect on IL-1β and IL-6 production, and it has no effect on TNF-α production. |
| Key words: cell hypoxia cerebral cortex interleukin-1beta interleukin-6 tumor necrosis factor-alpha |
