Objective To study whether stabilizing microtubules can decrease myocardial ischaemic-reperfusion injury. Methods (1) The isolated rat hearts were divided into four groups (n=15): control group, ischemic group, ischemic+0.1 μmol/L Taxol group, and ischemic+1 μmol/L Taxol group. All the groups were given a 15-min equilibration and then followed by different treatments: control group, 50 min normoxic superfusion; ischemia group, 20 min normoxic superfusion plus 30 min ischemia; and Taxol groups, 20 min normoxic superfusion plus 30 min ischemia, plus 0.1 or 1 μmol/L Taxol throughout 50-min superfusion period. Arrthymias scores were assessed and compared between different groups; the structure of the microtubules was observed and its breakage score was obtained. (2) The isolated rat hearts were divided into 3 groups (n=8): normal control group, ischemic/reperfusion (I/R) group, and 1 μmol/L Taxol group. The I/R group was Langendorff-perfused; the left anterior descending branch was ligated for 30 min and reperfused for 120 min. The Taxol group received 1 μmol/L Taxol and other treatments were similar to the I/R group. Evans blue perfusion was used to observe the infarct size of each group. Results Stabilizing microtubules with Taxol (0.1 μmol/L or 1 μmol/L) reduced ischaemic ventricular arrhythmia in a dose-dependent fashion (P<0.05); it also significantly reduced arrhythmia scores and the incidence of ventricular tachycardia (P<0.05). Taxol at 0.1 μmol/L greatly decreased microtubule breakage score, and at 1 μmol/L significantly reduced the infarct size compared with the control group (P<0.05). Conclusion Stabilizing microtubules can reduce myocardial ischaemic-reperfusion injury.