Objective To evaluate the laryngeal function recovery following laryngeal defect reconstruction with anterior cervical turnover myocutaneous flap after partial laryngectomies. Methods The swallowing, respiration and phonation functions of 43 patients, who received vertical partial laryngectomy, extended vertical partial laryngectomy, expanded frontovertical partial laryngectomy or subtotal laryngectomy, and laryngeal reconstruction with turnover myocutaneous flap between 2008 and 2010, were retrospectively analyzed. Thirty-two patients had glottic carcinoma (15 phase Ⅱ,16 phase Ⅲ, 1 phase Ⅳ) and 11 had transglottic carcinoma (5 phase Ⅱ,6 phase Ⅲ). The aspiration rate and decannulation rate were recorded. The swallowing function score, respiration function score, and voice parameters were analyzed and compared before and after operation. Results Six months after operation all the cases had normal swallowing and respiration functions, with no aspiration and with a decannulation rate of 100%. The swallowing function scores were similar before and after operation (U=903.1,P=0.559), and the respiratory function was significantly improved after operation (U=713.5,P=0.012). All the cases had normal voice. Six months after operation, the voice quality (such as Jitter, Shimmer and normalized noise energy \[NNE\]) were significantly better than that before the operation (P<0.05). The fundamental frequency (F0) of voice was significantly decreased after operation (P<0.05), and the NNE at >12 months after operation was significantly better than that at 6 months after operation (P<0.05). Conclusion Anterior cervical turnover myocutaneous flap for reconstruction of laryngeal defects following partial laryngectomies can achieve high decannulation rate and better phonation recovery, showing a satisfactory restoration of laryngeal function.