| 摘要: |
| 目的 观察白三烯B4受体1（leukotriene B4 receptor 1，LTB4-BLT1）拮抗剂U75302对脓毒症小鼠免疫功能的影响，探讨阻断白三烯B4受体1在脓毒症治疗中的意义。方法 采用盲肠结扎穿孔术（cecal ligation and puncture，CLP）致脓毒症小鼠模型，将18只雄性C57BL/6小鼠随机分为假手术组（n=6）、 CLP组（n=6）、CLP+腹腔注射U75302组（n=6）。手术后24 h检测3组小鼠外周血中肿瘤坏死因子α（tumor necrosis factor-α，TNF-α）及白介素10（interleukin-10，IL-10）的水平，利用流式细胞术检测3组小鼠腹腔灌洗液中Gr-1+细胞的计数以及胸腺T淋巴细胞的凋亡情况。结果 与CLP组相比，CLP+腹腔注射U75302组小鼠外周血中的TNF-α水平降低约43%（P<0.05），IL-10水平升高约88%（P<0.05），腹腔Gr-1+细胞计数降低（P<0.05），胸腺T淋巴细胞凋亡比例减少（P<0.01）。结论 LTB4-BLT1拮抗剂U75302可降低外周血TNF-α水平、提高IL-10水平、改善细胞免疫，可能干预脓毒症的炎症反应。 |
| 关键词: 脓毒症 白三烯B4受体1 白三烯拮抗剂 细胞免疫 炎症 |
| DOI：10.3724/SP.J.1008.2014.00246 |
| 投稿时间：2013-09-26修订日期：2013-11-29 |
| 基金项目: |
|
| Effects of U75302, blocker of leukotriene B4 receptor 1, on cellular immunity and inflammation in septic mice |
| LI Xiu-juan,WANG Jia-feng,ZHANG Yan,LI Jin-bao,DENG Xiao-ming* |
(Department of Anesthesiology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
*Corresponding author.) |
| Abstract: |
| Objective To observe the influence of U75302, blocker of leukotriene B4 receptor 1, on immune function in septic mice, so as to explore the implication of blocking leukotriene B4 receptor 1 for treatment of sepsis. Methods Experimental sepsis model was induced by cecal ligation and puncture (CLP). A total of 18 male C57BL/6 mice were randomly divided into sham group (n=6), CLP group (n=6) and CLP + U75302 intraperitoneal injection group (n=6). The peripheral blood cytokine tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) levels, peritoneal lavage fluid Gr-1+ cell count and thymus T lymphocytes apoptosis were detected in mice of three groups 24 h after surgery. Results Compared with the CLP group, CLP + U75302 intraperitoneal injection group had significantly reduced blood TNF-α level (by 43%, P<0.05), significantly increased IL-10 level (by 88%, P<0.05), signficantly decreased Gr-1+ cell counts in peritoneal lavage fluid (P<0.05), and significantly decreased apoptosis of thymus T lymphocytes (P<0.01). Conclusion Blocking leukotriene B4 receptor 1 with U75302 may decrease peripheral TNF-α level, increase IL-10 level, and improve cellular immunity, which may be involved in inflammation of sepsis. |
| Key words: sepsis leukotriene B4 receptor 1 leukotriene antagonists cellular immunity inflammation |
