| 摘要: |
| 目的 研究肠道补充低聚半乳糖（galacto-oligosaccharides，GOS）对冷束缚应激（cold restrain stress， CRS）大鼠肠黏膜紧密连接和屏障功能的影响。 方法 40只Wistar大鼠随机分成正常对照组、GOS组、CRS组和CRS+GOS组，每组10只，正常对照组仅给予基础饮食，GOS组每天给予GOS（4 g/kg）喂养，共10 d；CRS组大鼠于普通喂养第10天，被束缚固定后置于4℃冰箱3 h，制备CRS模型；CRS+GOS组在每天给予GOS（4 g/kg）喂养的第10天制备CRS模型。建模成功后，各组大鼠经心脏采血，检测血浆二胺氧化酶（diamine oxidas，DAO）浓度；处死大鼠，取回肠组织行病理检查及电镜检查，再采用免疫组化法检测细胞紧密连接蛋白occludin的表达，并以实时定量PCR法检测其在mRNA水平的表达。 结果 与正常对照组相比，CRS组及CRS+GOS组大鼠血浆DAO浓度均明显增高（P<0.01）；CRS组血浆DAO浓度又明显高于CRS+GOS组（P<0.01）；CRS组及CRS+GOS组大鼠肠黏膜occludin在蛋白和mRNA水平的表达均较正常对照组显著降低（P<0.01）；CRS组上述指标又明显低于CRS+GOS组（P<0.01），正常对照组与GOS组间上述指标的差异均无统计学意义（P>0.05）。 结论 添加GOS喂养可以降低大鼠血浆DAO浓度，增加回肠occludin 在mRNA和蛋白水平的表达，提示GOS可能具有改善紧密连接和维护肠屏障功能的作用。 |
| 关键词: 应激 肠黏膜 紧密连接 二胺氧化酶 occludin 低聚半乳糖 |
| DOI：10.3724/SP.J.1008.2014.00055 |
| 投稿时间：2013-09-26修订日期：2013-12-20 |
| 基金项目: |
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| Effect of prebiotics on intestinal barrier function in stressed rats |
| XIE Ting1, XIA Jin-rong1*, GAO Jun2, LI Zhao-shen2 |
(1. Department of Gastroenterology, Zhongda Hospital, Southeast University, Nanjing 210009, Jiangsu, China;
2. Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
*Corresponding author.) |
| Abstract: |
| Objective To evaluate the effect of the galacto-oligosaccharides (GOS) on the intestinal mucosal tight junction and barrier function of rats with induced cold restraint stress. Methods Forty Wistar rats were randomly divided into four groups: two cold resistant stress (CRS) groups fed with either a standard diet (CRS group) or 4 g/kg GOS per day (CRS+GOS group) and two sham CRS groups fed with a standard diet (control group) or 4 g/kg GOS per day (GOS group). All the four groups were fed for a total of 10 days, and the CRS and CRS+GOS groups were subjected to cold restraint stress on the 10th day. Then the blood samples of all the four groups were collected via cardio puncture for determination of serum diamine oxidas (DAO) activity; finally all the rats were sacrificed and the intestinal tissues were collected for pathological and electronic microscopic examination, with the occludin mRNA level measured by real-time PCR and the occludin protein expression by immune-histological method. Results Compared with the control, the serum DAO levels of CRS group and the CRS+GOS group were significantly increased(P<0.01); and that in the CRS group was significantly higher than that in the CRS+GOS group (P<0.01). The levels of occludin mRNA and protein expression were significantly decreased in the two CRS groups (P<0.01), and that in the CRS group was significantly lower than that in the CRS+GOS group (P<0.01); the serum DAO levels, the expressions of occludin mRNA and protein were not significantly different between the control and the GOS groups. Conclusion Feeding with GOS can decrease plasma level of DAO in rats, increase expression of intestinal occludin mRNA and protein in rat ileum, indicating that GOS can improve the mucosal tight junction and intestinal barrier function. |
| Key words: stress intestinal mucosa tight junctions diamine oxidase occludin galacto-oligosaccharides |
