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  • 马永强,王振国,苟学立,李娜,冯亚强,柳文娟,刘斌,李志革.石墨烯量子点对小鼠小肠成像及微循环血流的影响[J].第二军医大学学报,2014,35(4):372-377    [点击复制]
  • MA Yong-qiang,WANG Zhen-guo,GOU Xue-li,LI Na,FENG Ya-qiang,LIU Wen-juan,LIU Bin,LI Zhi-ge.Effect of graphene quantum dots on intestinal imaging and mesenteric microcirculation in mice[J].Acad J Sec Mil Med Univ,2014,35(4):372-377   [点击复制]
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石墨烯量子点对小鼠小肠成像及微循环血流的影响
马永强1,2,王振国1,苟学立1,李娜1,冯亚强2,柳文娟1,刘斌1,李志革3*
0
(1. 兰州大学口腔医学院修复教研室, 兰州 730000;
2. 中国科学院兰州化学与物理研究所固体润滑国家重点实验室, 兰州 730000;
3. 兰州大学口腔医学院外科学教研室, 兰州 730000
*通信作者)
摘要:
目的 研究石墨烯量子点(GQDs)对小鼠小肠的成像性能及对小鼠肠系膜微循环的影响。方法 以纯水和碲化镉量子点(CdTe QDs)作为阴性及阳性对照,并与临床上常用的钡餐作对比,采用FX-in vivo小动物活体成像系统进行GQDs对C57BL/6N小鼠小肠的成像研究,用微循环显微镜观察其对肠系膜微动、静脉和毛细血管的影响。结果 GQDs 灌胃1 h后紫外光激发下小鼠小肠呈亮绿色充盈影像,肠壁边缘形态及黏膜细微结构清楚,肠系膜血管呈淡绿色影像;钡餐灌胃1 h后X线照射下小肠呈高密度团块影像,肠壁黏膜细微结构不清楚,不能显示肠系膜血管。与灌胃前比,GQDs灌胃后小鼠肠系膜微动脉管径增大,而钡餐灌胃后微动脉管径减小;GQDs、CdTe QDs、钡餐灌胃对小鼠肠系膜微静脉管径均无明显影响;钡餐和CdTe QDs灌胃后小鼠肠系膜微动、静脉及毛细血管的血流速度加快,而GQDs灌胃对小鼠肠系膜微动、静脉及毛细血管的血流速度则无明显影响。结论 GQDs和钡餐均能对C57BL/6N小鼠小肠成像,GQDs对肠壁及黏膜的成像效果优于钡餐,对小鼠肠系膜微循环的影响较小。
关键词:  石墨烯量子点  硫酸钡  肠系膜  微循环  小肠成像
DOI:10.3724/SP.J.1008.2014.00372
投稿时间:2013-12-01修订日期:2014-01-03
基金项目:甘肃省科技支撑项目(1304FKCA074);兰州大学口腔医学院科研扶持基金(533000-432363).
Effect of graphene quantum dots on intestinal imaging and mesenteric microcirculation in mice
MA Yong-qiang1,2,WANG Zhen-guo1,GOU Xue-li1,LI Na1,FENG Ya-qiang2,LIU Wen-juan1,LIU Bin1,LI Zhi-ge3*
(1. Department of Prosthodontics, School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu, China;
2. State Key Laboratory of Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, Gansu, China;
3. Department of Oral and Maxillofacial Surgery, School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu, China
*Corresponding author.)
Abstract:
Objective To study the effect of graphene quantum dots (GQDs) on intestinal image formation and mesenteric microcirculation in mice. Methods The effect of GQDs on intestinal image formation was observed using FX-in vivo imaging system in C57BL/6N mice by comparing with X-ray barium meal examination. Pure water was used as negative control and CdTe QDs was used as positive control. The effects of GQDs on the mesenteric arterioles, venules, and capillaries were observed by the mesenteric microcirculation microscope. Results The mouse intestine took on a bright green image under ultraviolet stimulation 1 h after receiving gavage with GQDs, with clear edge and mucosal structure; meanwhile, mesenteric vessels were in clear light green in the mice. High-density images of the small intestine were observed by X-ray in barium meal examination, but the intestinal mucosal structure was not clear, and the mesenteric vessels were not displayed. The diameter of small arteries in GQDs group was greater and that in the barium meal group was smaller than that before gavage. GQDs, CdTe QDs and barium meal had no notable impact on mouse mesenteric venules. The velocities of blood flow in mesenteric arterioles, venules and capillaries were increased after the administration of barium meal or CdTe QDs, and there were no significant changes in GQDs group. Conclusion GQDs is superior to barium meal in image formation of mouse intestinal wall, and it has less influence on mouse mesenteric microcirculation.
Key words:  graphene quantum dots  barium sulfate  mesentery  microcirculation  intestinal imaging