| 摘要: |
| 目的 探讨三维球体胎盘来源间充质干细胞(MSCs)移植对大鼠缺血再灌注损伤脑组织Nogo-A及其受体NgR表达的影响。 目的 大鼠随机分为假手术组(Sham组)、溶剂对照组(Vehicle组)及治疗组(MSCs组),后两组应用线栓法制备大脑中动脉缺血模型,缺血2 h后拔除鱼线再灌注,1 d后MSCs组注射三维球体MSCs,Vehicle组注射等量培养液;移植后1、3、7 d测定大鼠神经运动功能,取脑组织,应用RT-PCR法、蛋白质印迹法检测Nogo-A、NgR mRNA及蛋白表达。 结果 与Vehicle组相比,MSCs组大鼠7 d神经运动功能有明显改善(P<0.05);移植后1、3、7 d MSCs组Nogo-A、NgR mRNA及蛋白水平均较Vehicle组降低(P<0.05)。 结论 三维球体MSCs移植可改善脑缺血再灌注后神经运动功能,其机制可能与下调脑组织Nogo-A及其受体NgR水平有关。 |
| 关键词: 脑缺血 再灌注损伤 间质干细胞移植 Nogo-A NgR |
| DOI：10.3724/SP.J.1008.2015.01087 |
| 投稿时间：2015-01-04修订日期：2015-03-26 |
| 基金项目:全军医学科研"十二五"重点课题(BWS11J003). |
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| Effect of transplantation with the three-dimensional spheroid-cultured mesenchymal stem cells on Nogo-A and NgR expression in rats with cerebral ischemia reperfusion injury |
| LIU Ming1,XIA Yu-jun1,ZHANG Ming1,FU Qing-song1,SHAN Shou-qin2*,WANG Hong-xia3 |
(1. Department of Anatomy, Medical College of Qingdao University, Qingdao 266071, Shandong, China;
2. Qingdao First Sanatorium, PLA Jinan Military Area Command, Qingdao 266071, Shandong, China;
3. Department of Neurology Emergency, The Affiliated Hospital of Qingdao University, Qingdao 266071, Shandong, China
*Corresponding author.) |
| Abstract: |
| Objective To investigate the effect of transplantation with the three-dimensional spheroid-cultured mesenchymal stem cells (MSCs) on the expression of Nogo-A and NgR in rats with cerebral ischemia-reperfusion injury. Methods The experimental animals were randomly divided into Sham group, Vehicle group and MSCs treated group. The model of focal ischemia-reperfusion in rats was induced by intraluminal middle cerebral artery (MCA) occlusion with a nylon monofilament suture in Vehicle group and MSCs treated group. The fishing line was unpluged for reperfusion 2 h after ischemia and MSCs were transplanted in MSCs treated group one day later. Equivalent medium solution was given to the Vehicle group 1 d later. On the 1st day, 3rd day, and 7th day after transplantation, the neuromotor function of the animals was detected. The brain tissue of rats was harvested for RT-PCR detection of Nogo-A and NgR mRNA expression in the brain tissue of rats, and Western blotting analysis was used to detect the expression of Nogo-A and NgR protein. Results Compared with the Vehicle group, the neuromotor function was significantly improved in MSCs treated group on the 7th day; and the expressions of Nogo-A and NgR mRNA and protein were significantly down-regulated in MSCs treated group on the 1st day, 3rd day, and 7th day after transplantation (P<0.05). Conclusion Transplantation of the three-dimensional spheroid-cultured MSCs can improve the neuromotor function following cerebral ischemia/reperfusion injury, and its mechanism may be associated with down-regulation of Nogo-A and NgR in the brain tissue. |
| Key words: brain ischemia reperfusion injuries mesenchymal stem cell transplantation Nogo-A NgR |
