| 摘要: |
| 兰尼碱受体（ryanodine receptor， RyR）是位于肌浆网膜上的细胞内Ca2+释放通道，在骨骼肌和心肌兴奋收缩偶联等生理过程中发挥重要作用。随着单粒子冷冻电镜技术的应用以及数据分析能力的提高，近期来自中国、美国以及德国的3个课题组分别获得了整体分辨率为3.8Å （1Å=10-10m）、4.8Å和6.1Å的高清晰RyR1结构图片，相关研究同时发表于2015年第1期的Nature上，是近年来RyR结构及其门控研究的重要进展。RyR1为相对分子质量>2200000的同源四聚体离子通道，主要包括由NTD、SPRY、P1、P2、B-sol以及C-sol等结构域组成的胞质区和由S1~S6、VSL以及CTD等结构域组成的通道区。Ca2+作为RyR1门控的主要影响因子，能够与胞质区EF-hand亚结构域结合，引起通道构象的变化并最终导致通道的开放。 |
| 关键词: 兰尼碱受体 钙 通道 冷冻电镜 结构 |
| DOI：10.16781/j.0258-879x.2016.07.0873 |
| 投稿时间：2016-03-15修订日期：2016-06-07 |
| 基金项目:国家自然科学基金面上项目（81470518），2015年度第二军医大学大学生创新基金面上项目（MS2015014，MS2015028）. |
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| Ryanodine receptor 1 and its potential gating mechanism: recent progress |
| GU Jia-wei1,DENG Yu-chen2,XU Zheng3,XIAO Liang4* |
(1. No. 11 Student Team, Clinical Medicine, Second Military Medical University, Shanghai 200433, China;
2. No.;
3 Student Team, Naval Clinical Medicine, Second Military Medical University, Shanghai 200433, China;
3. Division of Scientific Research Administration, Second Military Medical University, Shanghai 200433, China;
4. Department of Marine Biotechnology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China
* Corresponding author) |
| Abstract: |
| The ryanodine receptors (RyRs) are intracellular Ca2+ releasing channels on the sarcoplasmic reticulum membrane and play a pivotal role in the excitation-contraction coupling of skeletal and cardiac muscles as well as other physiological processes. With the recent development of Cryo-EM and the improvement of data analysis technique, scientists from China, the United States and Germany have acquired high-quality RyR1 images at the total resolutions of 3.8 Å (1 Å=10-10 m), 4.8 Å and 6.1 Å, respectively, which have been published in the same issue of Nature in 2015. RyR1s are homotetrameric complexes with a molecular mass of more than 2200000, mainly containing a cytoplasmic region composed of NTD, SPRY, P1, P2, B-sol and C-sol domains and a channel region composed of S1-S6, VSL and CTD domains. As the most common factor affecting the condition of RyR1, Ca2+ is able to bind the EF-hand subdomain in the cytoplasmic region, which further causes the conformational change and finally leads to the channel opening. |
| Key words: ryanodine receptor calcium channel cryo-electronic microscopy structure |
