Objective To observe the changes of uncorrected visual acuity, corneal curvature, corneal thickness and axial length in adolescent patients with myopia after wearing night-wear orthokeratology lens (Ortho-KCL). Methods Fifty adolescent patients (100 eyes) with degree of myopia <-6.00 D in Changzheng Hospital of Second Military Medical University were treated by wearing night-wear orthokeratology lens (Autek China Inc.) from Jun. 2010 to Jun. 2012. The uncorrected visual acuities of patients were measured by phoropter before treatment and on 3^rd, 6^th, 12^th and 24^th months after treatment, the corneal curvature and corneal thickness was measured by corneal topography, and the axial length was measured by A/B ultrasonic diagnostic system. Results The ucorrected visual acuities of patients at 3, 6, 12 and 24 months after treatment were increased compared with that before treatment, and the average K values of corneal curvature were significantly decreased (all P<0.01); the axial lengths were slightly increased, but the differences were not statistically significant (all P>0.05). There was no significant difference in corneal thickness of patients before and after treatment. Conclusion Night-wear orthokeratology can improve the uncorrected vision of adolescent patients with myopia, and can effectively and safely slow down the progression of myopia.