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| 可同时释放两种骨形态发生蛋白复合支架的制备 |
| 卢春闻1,王超1,袁佳滨1,丁慕晨1,徐晓博2,石志才1*,毛宁方1* |
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(1. 第二军医大学长海医院骨科, 上海 200433;
2. 北方战区陆军65571部队卫生队, 四平 136000
*通信作者) |
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| 摘要: |
| 目的 研制一种可同时释放骨形态发生蛋白2（BMP-2）和骨形态发生蛋白7（BMP-7）的生物活性复合支架，以应用于骨组织工程的研究。方法 分别采用复乳溶剂挥发法和相分离法制备负载BMP-2和BMP-7的聚乳酸/羟基乙酸-聚乙二醇（PGLA-PEG）微球和三维多孔的聚己内酯（PCL）支架。然后采用改良的二氯甲烷熏蒸法将PGLA-PEG微球黏附在PCL支架上，形成同时负载BMP-2和BMP-7的复合支架，并检测BMP-2和BMP-7的缓释效果。将人成骨细胞系hFOB1.19分别种植在复合支架和传统支架中，研究支架上的细胞增殖能力和成骨分化能力。结果 制备的复合支架能同时缓慢地释放BMP-2和BMP-7。细胞培养第10天，复合支架上的细胞活性优于传统支架（P<0.01），细胞形态正常。复合支架上细胞的碱性磷酸酶和Ⅰ型胶原蛋白、骨钙素、骨桥蛋白 3种成骨基因的mRNA水平均高于传统支架（P<0.05，P<0.01）。结论 成功研制出一种可同时释放BMP-2和BMP-7的生物活性复合支架，并可用于骨组织工程的研究。 |
| 关键词: 骨和骨组织 组织工程 骨形态发生蛋白质类 聚合物 组织支架 骨生成 |
| DOI：10.16781/j.0258-879x.2017.02.0188 |
| 投稿时间：2016-06-21修订日期：2017-01-11 |
| 基金项目:国家自然科学基金（81272026，8167090274）. |
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| Bone tissue engineering of a composite scaffold simultaneously releasing two kinds of bone morphogenetic proteins |
| LU Chun-wen1,WANG Chao1,YUAN Jia-bin1,DING Mu-chen1,XU Xiao-bo2,SHI Zhi-cai1*,MAO Ning-fang1* |
(1. Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;
2. Medical Team, No. 65571 Troop of PLA Northern Theater Command, Siping 136000, Jilin, China
*Corresponding authors) |
| Abstract: |
| Objective To prepare a 3D polymer scaffold capable of simultaneously releasing both bone morphogenetic protein (BMP)-2 and BMP-7 for osteogenic differentiation of hFOB1.19 cells in bone tissue engineering. Methods The BMP-2 and BMP-7 microspheres of poly(glycolic acid-co-lactic acid)-polyethylene glycol (PGLA-PEG) were prepared using an emulsion method, and the 3D porous scaffold of PGLA-PEG was produced by phase separation. Then in order to obtain composite scaffold loaded with BMPs, the two microspheres were fixed into scaffolds with methylene chloride fumigation technology, and the slow-release of BMP-2 and BMP-7 was observed. The human osteoblast line cells (hFOB1.19) were seeded on the prepared composite scaffolds for testing cell proliferation and osteogenic differentiation. Results It was showed that the produced 3D composite scaffolds could release the BMP-2 and BMP-7 gradually. The hFOB1.19 cells grew better on the 3D composite scaffolds than on the traditional scaffolds on day 10 of cell culture (P<0.01), and the cells had normal morphology. The mRNA levels of alkaline phosphatase, collagen type-Ⅰ, osteocalcin and osteopontin of cells on the 3D composite scaffolds were higher than those on the scaffold without BMPs (P<0.05, P<0.01). Conclusion We have successfully prepared a 3D polymer scaffold which can simultaneously release BMP-2 and BMP-7, and it may be used bone for tissue engineering. |
| Key words: bone and bones tissue engineering bone morphogenetic proteins polymers tissue scaffolds osteogenesis |
