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赵婧,魏品康,修丽娟,矫健鹏,俞超芹.消痰解郁方对乳腺癌前病变MCF-10AT细胞PI3K/Akt通路的影响及机制[J].第二军医大学学报,2017,38(4):520-523 [点击复制]
ZHAO Jing,WEI Pin-kang,XIU Li-juan,JIAO Jian-peng,YU Chao-qin.Effect of Xiaotan Jieyu Decoction on PI3K/Akt pathway of MCF-10AT cells and its mechanism[J].Acad J Sec Mil Med Univ,2017,38(4):520-523 [点击复制]

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消痰解郁方对乳腺癌前病变MCF-10AT细胞PI3K/Akt通路的影响及机制
赵婧¹,魏品康¹,修丽娟¹,矫健鹏¹,俞超芹^2*
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(1. 第二军医大学长征医院中医科, 上海 200003; 2. 第二军医大学长海医院中医科, 上海 200433 ^*通信作者)

摘要:

目的探讨消痰解郁方对乳腺癌前病变MCF-10AT细胞PI3K/Akt信号通路的影响及机制。方法将MCF-10AT细胞分为消痰解郁方组、PI3K激酶选择性抑制剂LY294002组（抑制剂组）、消痰解郁方+抑制剂组和对照组。给予相应药物干预24、48 h后，采用CCK-8法观察各组细胞增殖及生长情况；药物干预48 h后，采用流式细胞仪检测各组细胞周期变化，用蛋白质印迹法检测各组细胞PTEN、PI3K、Akt蛋白表达变化。结果药物干预24和48 h后，消痰解郁方组、抑制剂组和消痰解郁方+抑制剂组MCF-10AT细胞增殖受到抑制，且消痰解郁方+抑制剂组抑制率高于消痰解郁方组和抑制剂组，差异有统计学意义（P<0.05）；药物干预48 h后，消痰解郁方组、抑制剂组和消痰解郁方+抑制剂组MCF-10AT细胞G₀/G₁期细胞比例增高，S期及G₂/M期细胞比例下降，与对照组比较差异有统计学意义（P<0.05），其中消痰解郁方+抑制剂组与其他各组相比差异有统计学意义（P<0.05）；药物干预48 h后，消痰解郁方组、抑制剂组、消痰解郁方+抑制剂组PI3K、p-Akt 蛋白表达较对照组减弱，PTEN蛋白表达较对照组增强，差异均有统计学意义（P<0.05）。结论消痰解郁方可能通过抑制PI3K/AKT信号通路发挥其对MCF-10AT细胞的抑制及促凋亡作用。

关键词: 乳腺肿瘤 MCF-10AT细胞 PI3K/Akt信号通路细胞凋亡

DOI：10.16781/j.0258-879x.2017.04.0520

投稿时间：2017-02-10修订日期：2017-03-13

基金项目:上海市名老中医学术经验研究工作室建设项目（ZYSNXD-CC-MZY054），军队中医药科研专项基金（2010z131）.

Effect of Xiaotan Jieyu Decoction on PI3K/Akt pathway of MCF-10AT cells and its mechanism

ZHAO Jing¹,WEI Pin-kang¹,XIU Li-juan¹,JIAO Jian-peng¹,YU Chao-qin^2*

(1. Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;
2. Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medicine University, Shanghai 200433, China
^*Corresponding author)

Abstract:

Objective To study the effect of Xiaotan Jieyu (XTJY) Decoction on PI3K/Akt signal pathway of MCF-10AT cells of breast precancerous lesion and the ralated mechanism. Methods The MCF-10AT cells were randomly divided into following groups: XTJY group, PI3K/Akt inhibitor LY294002 (LY) group, XTJY+LY group and control (CON) group. After corresponding drug intervention, CCK-8 method was used to observe the MCF-10AT cell proliferation and growth inhibition at 24 h and 48 h, flow cytometry was used to detect the changes of cell cycle at 48 h, and the expression changes of PTEN, PI3K and Akt protein were detected by Western blotting analysis at 48 h. Results The proliferation of MCF-10AT cells were significantly inhibited at 24 h and 48 h in XTJY, LY and XTJY+LY groups, and the inhibitory rate of MCF-10AT cells in XTJY+LY group was significantly higher than those in XTJY and LY groups (P<0.05). Compared with CON group, the percentages of G₀/G₁ phase cells in XTJY, LY and XTJY+LY groups were significantly increased at 48 h (P<0.05), while the percentages of S phase and G₂/M phase cells were significantly decreased (P<0.05); besides, there were significant differences between XTJY+LY group and the other two groups (P<0.05). Compared with CON group, the expressions of PI3K and p-AKT protein in XTJY, LY and XTJY+LY groups were significantly decreased at 48 h (P<0.05), and the expression of PTEN protein was significantly increased (P<0.05). Conclusion XTJY prescription may exert inhibitory and apoptotic effect on MCF-10AT cells through inhibiting the PI3K/Akt signal pathway.

Key words: breast neoplasms MCF-10AT cells PI3K/Akt signal pathway apoptosis