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  • 胡婷,王萍.新型三重抗生素联合氢氧化钙对粪肠球菌的体外抗菌效果[J].第二军医大学学报,2017,38(12):1601-1605    [点击复制]
  • HU Ting,WANG Ping.Antimicrobial efficacy of new triple antibiotics in combination with calcium hydroxide for Enterococcus faecalis: an in vitro comparative study[J].Acad J Sec Mil Med Univ,2017,38(12):1601-1605   [点击复制]
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新型三重抗生素联合氢氧化钙对粪肠球菌的体外抗菌效果
胡婷,王萍*
0
(重庆医科大学附属第一医院口腔科, 重庆 400016
*通信作者)
摘要:
目的 观察新型三重抗生素联合氢氧化钙对根管内粪肠球菌感染的体外抗菌效果。方法 选取80颗牙体完整、发育正常的离体人单根管前磨牙,建立粪肠球菌离体根管感染模型。将其随机分为5组,每组16颗,分别使用生理盐水(NS组)、氢氧化钙(CH组)、传统三重抗生素(甲硝唑、米诺环素和环丙沙星,TAP组)、新型三重抗生素(奥硝唑、米诺环素和环丙沙星,NTAP组)和新型三重抗生素联合氢氧化钙(NTAP+CH组)进行根管内封药,采用菌落计数法分别观察封药后第3天和第7天的粪肠球菌菌落数并利用扫描电镜观察。结果 封药后第3、7天,NTAP组和NTAP+CH组的细菌菌落数均低于TAP组和CH组(P<0.05)。NTAP+CH组封药后第3天的细菌菌落数较NTAP组减少(P<0.05),而在第7天时两组差异无统计学意义(P>0.05)。扫描电镜下可见封药后第3、7天,NTAP组和NTAP+CH组的根管壁上粪肠球菌的菌落数较其余3组减少。结论 新型三重抗生素单独应用或联合氢氧化钙应用对粪肠球菌的抗菌效果均优于传统三重抗生素和氢氧化钙,可以认为新型三重抗生素和新型三重抗生素联合氢氧化钙是针对根管粪肠球菌感染的有效消毒药物。
关键词:  肠球菌  抗生素  氢氧化钙  根管内封药  生物膜
DOI:10.16781/j.0258-879x.2017.12.1601
投稿时间:2017-05-21修订日期:2017-07-03
基金项目:重庆市卫生和计划生育委员会医学科研项目重点项目(20141001).
Antimicrobial efficacy of new triple antibiotics in combination with calcium hydroxide for Enterococcus faecalis: an in vitro comparative study
HU Ting,WANG Ping*
(Department of Stomatology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
*Corresponding author)
Abstract:
Objective To evaluate the antibacterial effects of the combination of calcium hydroxide and new triple antibiotics against Enterococcus faecalis (E. faecalis). Methods Eighty single rooted premolar teeth (intact and normal development) were included in this study. E. faecalis infected model was established in vitro. Model teeth were divided randomly into five groups with 16 teeth in each group, and the teeth in the five groups were treated using normal saline (NS group), calcium hydroxide (CH group), conventional triple antibiotics (mixed with metronidazole, minocycline and ciprofloxacin, TAP group), new triple antibiotics (mixed with ornidazole, minocycline and ciprofloxacin, NTAP group) or new triple antibiotics in combination with calcium hydroxide (NTAP+CH group), respectively, as intracanal medicament. The colony-forming units were counted at day 3 and day 7 after operation using colony-counting method. Scanning electron microscope was used to confirm the bacterial counting results. Results The colony-forming units on day 3 and day 7 of post-operation in the NTAP and NTAP+CH groups were both significantly lower than those in the TAP and CH groups (both P<0.05). Compared with the NTAP group, the NTAP+CH group showed a significant decrease in colony-forming units on day 3 of post-operation (P<0.05), but there was no significant difference on day 7 between the two groups (P>0.05). Scanning electron microscope results showed fewer bacteria on the root canal walls in the NTAP and NTAP+CH groups than the other three groups. Conclusion Antibacterial effect of new triple antibiotic used alone or in combination with calcium hydroxide for E. Faecalis is superior to conventional triple antibiotics and calcium hydroxide. So new triple antibiotic used alone or in combination with calcium hydroxide can be considered as an effective alternative intracanal medicament.
Key words:  Enterococcus  antibiotics  calcium bydroxide  intracanal medicament  biomembrane