Objective To explore the effects of microRNA-484 (miR-484) on hepatic stellate cells (HSCs), the key cell in the occurrence and development of liver fibrosis, and to investigate the functions. Methods On the basis of our previous microarray analysis results, we transfected HSC-T6 cell lines with miR-484 inhibitor to intervene the expression of miR-484 in vitro. The expressions of mRNA and proteins related to liver fibrosis and apoptosis were detected by qPCR and Western blotting, respectively. The cell apoptosis with Annexin Ⅴ-FITC/PI double staining was determined by flow cytometry. Results Compared with the control group, the mRNA and protein expression of miR-484-targeted gene Fis1 and proapoptotic factor caspase-3 were both significantly up-regulated (P<0.05), and apoptosis inhibitory factor Bcl-2 was significantly down-regulated (P<0.05) in the HSC-T6 cells transfected with miR-484 inhibitor; the apoptosis rate of HSC-T6 cells was significantly increased ([32.81±3.21]% vs[57.54±6.76]%, P<0.05), and α-smooth muscle actin and collagen type Ⅰ were both significantly down-regulated (P<0.05) in the HSC-T6 cells transfected with miR-484 inhibitor. Conclusion MiR-484 promotes the occurrence and progress of liver fibrosis through inhibiting the apoptosis and promoting the activation of HSCs by targeting Fis1.