Exosomes are the microvesicles released from cells during fusion of multivesicular endosomes with plasma membrane. In recent years, most studies have focused on the function of some molecules contained in exosomes, such as messenger RNA (mRNA), microRNA and long intergenic non-coding RNA (lincRNA). The studies about glycoproteins contained in exosomes are rarely reported. Recent researches have shown that many diseases, including tumor, are closely associated with glycoproteins contained in exosomes. In this review, we described the role of exosome glycoproteins in tumors and other diseases.