| 摘要: |
| 目的 建立高效液相色谱（HPLC）梯度洗脱方法同时测定瑞香狼毒中狼毒色原酮、新狼毒素A、狼毒素、异狼毒素及7-甲氧基新狼毒素A 5种黄酮类成分的含量。方法 采用HPLC-二极管阵列检测器（HPLC-DAD）法，色谱柱为Agilent Eclipse Plus C18 （3.0 mm×100 mm，3.5 μm）；流动相A为0.1%甲酸水溶液，流动相B为纯乙腈，洗脱梯度为0～6 min 20% B、6～10 min 20%～35% B、10～50 min 35% B；流速为0.4 mL/min；平衡时间设置为10 min；采集时间为50 min；柱温为25℃；DAD检测波长为290 nm；进样量为5 μL。结果 狼毒色原酮、新狼毒素A、狼毒素、异狼毒素及7-甲氧基新狼毒素A在该条件下分离良好，分别在4.680～468.0、2.016～201.6、3.784～378.4、5.520～552.0、0.974～97.40 μg/mL浓度范围内线性关系良好，且其精密度、稳定性、重复性和回收率均良好。结论 HPLC-DAD法稳定性、重复性好，可用于测定瑞香狼毒中狼毒色原酮、新狼毒素A、狼毒素、异狼毒素及7-甲氧基新狼毒素A 5种黄酮类成分的含量。 |
| 关键词: 高效液相色谱法 狼毒色原酮 新狼毒素A 狼毒素 异狼毒素 7-甲氧基新狼毒素A |
| DOI：10.16781/j.0258-879x.2018.11.1235 |
| 投稿时间：2018-05-24修订日期：2018-06-12 |
| 基金项目:国家自然科学基金青年科学基金（81303300）. |
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| High-performance liquid chromatography-diode array detector in determination of five flavonoids in Stellera chamaejasme L. |
| CHEN Jun,DU Hong-li,ZHOU Jin,LÜ Lei,LI Cheng-jian,PAN Li-ming,ZHAO Liang* |
(Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Navy Medical University(Second Military Medical University), Shanghai 200438, China
*Corresponding author) |
| Abstract: |
| Objective To develop a high-performance liquid chromatography (HPLC) method for simultaneous determination of five flavonoids (chamaechromone, neochamaejasmin A, chamaejasmine, isochamaejasmin and 7-methoxylneochaejasmin A) in the extraction of Stellera chamaejasme L.. Methods An HPLC-diode array detector (DAD) method was established. The HPLC-DAD condition was as follows:chromatographic column was Agilent Eclipse Plus C18 (3.0 mm×100 mm, 3.5 μm); mobile phase A was 0.1% formic acid-water solution; mobile phase B was acetonitrile; gradient of acetonitrile was 20% (0-6 min), 20%-35% (6-10 min) and 35% (10-50 min); flow rate was 0.4 mL/min; equilibration time was 10 min; acquisition time was 50 min; column temperature was 25℃; DAD detection wavelength was 290 nm; and sample injection volume was 5 μL. Results Chamaechromone, neochamaejasmin A, chamaejasmine, isochamaejasmin and 7-methoxylneochaejasmin A were successfully separated using this method, with good linear relationship between 4.680-468.0, 2.016-201.6, 3.784-378.4, 5.520-552.0 and 0.974-97.40 μg/mL, respectively. The precision, stability, repeatability and recovery of the five flavonoids were good with this method. Conclusion HPLC-DAD has good stability and repeatability, and can be used to determine the contents of five flavonoids (chamaechromone, neochamaejasmin A, chamaejasmine, isochamaejasmin and 7-methoxylneochaejasmin A) in Stellera chamaejasme L.. |
| Key words: high-performance liquid chromatography chamaechromone neochamaejasmin A chamaejasmine isochamaejasmin 7-methoxylneochaejasmin A |
